Nudt7 (BC069843) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300007B24Rik; 2210404C19Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC069843 GCTTCCCGAAGTCTCCTGCTGCTAGTAAGAGGAAGTACTGCAGGGCTGATGTCGCGACCTTGTGGACTCC CGGAGCCTGTCAGAAACAATCTGATAGATGATGCCAAGGCTCGCCTGAGAAAGTCTGATGTCGGGACCAG ATATTCTCACCTGTCCTCTAATAAATTCTCTGTCCTTGTACCACTGCTGGCCAGAGGAGGAAAGTTGTAT TTGATGTTCACGGTCCGCTCAGACAAGCTGAAAAGGGAACCTGGAGAAGTCTGCTTCCCTGGAGGAAAGA GGGACCCGGTGGACACAGATGACACAGCCACTGCTCTCCGTGAAGCCCAGGAGGAGGTGGGGCTGCATCC CCACCAAGTGGAGGTGGTCTCTCACCTGGTGCCATACGTATTTGATAATGATGCACTGGTAACCCCCGTA GTGGGTTTTCTAGACCACAACTTCCAGGCCCAACCTAATGCTGATGAAGTGAAGGAAGTCTTCTTTGTGC CTTTGGACTATTTCCTCCATCCCCAAGTCTACTACCAGAAGCAAATCACACAGTCTGGCCGTGATTTCAT CATGCATTGCTTCGAGTACAAAGACCCTGAGACTGGTGTGAACTACCTAATCCAGGGAATGACCTCAAAA CTGGCTGTGTTGGTGGCCTTAATTATTTTGGAACAAAGTCCTGCCTTCAAGATTGATTTTGATCTCCATG ACCTGATACCGTCTTGTGAAAGGACCTTCCTTTGGAGATATTCTTTAAGCAAGTTGTGAAGACCCTGAAA CCCAAAAGAACGTACATGAGGTTTTTGTGTGAGCTTATGCTCAGAACAACAGTGTTTAGCAAGTGGCATC TGACAGGTGTGAATATTTTCCTACAATGTGGCTGTGAGATCCTTGCCTGACTTCCTGTTGCTGCTTTAGA ACACTAAAAGTCTTTCAAAACTGGAAAAATGCACTGGCAGGGGAGAATAATTTTACCCATTTAAAAAAAT GGCACGATTTTTAGTCATATTAGGGATATCTGTCACATAGTAGGCACTTAATAAATATTTGTCAAATAAA AAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC069843
Insert Size	711 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC069843, AAH69843
RefSeq Size	1069 bp
RefSeq ORF	711 bp
Locus ID	67528
Gene Summary	Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. CoA into 3',5'-ADP and 4'-phosphopantetheine. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.[UniProtKB/Swiss-Prot Function]

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