Zw10 (BC012435) Mouse Untagged Clone
CAT＃: MC206234
Zw10 (untagged) - Mouse ZW10 homolog (Drosophila), centromere/kinetochore protein (cDNA clone MGC:11681 IMAGE:3711269), (10ug)
CNY 5712.00
货期*
现货
规格
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6330566F14Rik; MmZw10 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC012435
CGCTGGCAGCGGGGTTGACCTATCCTGTTTGTGTGGGTACGCTTCGTGGCGATGGCCTCTTTCGTGACAG AAGTCTTGGCCCACTCGGGGAGCCTGGAGAAGGAGGATCTGGGCACCAGGATCAGCCGCCTGACCCGGCG GGTGGAGGAGATCAAGGGTGAAGTATGCAATATGATCAGCAAGAAGTACAGTGAGTTCCTGCCTACCATG CAGAGTGCGCAGGCCCTAGTTACCCAGGTGGACACTCTATCTAACGACATCGACCAGCTGAAATCCAGGA TAGAGACTGAGGTCTGTCGGGATCTTCACATATCAACTGTTGAGTTTACAAACCTGAAGCAGCAGCTGGA AAGAGACTCTGTAGTCCTAACTCTGCTCAAACAACTCCAAGAGTTTTCCTCTGCTATTGAAGAGTATAAC AGTGCGTTAGCAGAGAAGAAGTACATCCCTGCTGCTCGGCACCTGGAGGAGGCTCAGGAGTGCTTGAAGT TATTAAAGTCCAGAAAATGCTTTGACTTAAAAATGTTGAAGTCTCTCAGCATGGAGCTCACCGTGCAGAA GCAGAACATACTGTACCACCTGGGAGAAGACTGGCAGAAGCTGGTTGTATGGAAGTTTCCGCCGGCAAAA GATACCAGCAGCTTAGAGTCTTGTCTACAAACAGAGCTTCATTTATGCACTGAGCAGCCGGAGAAAGAGG ACATGACTCCTCTGCCGTCCATCAGTTCTGTCCTCTTGGCCTTTTCCATTCTTGGAGAATTACCCACCAA GCTGAAGTCATTTGGTCAGATGCTGCTGAAGTATATCCTTAAGCCTCTGGTGACCTGCCCGTCGCTCCAT GCTGTGATTGAAAGGCAGCCAAGTTCAGTTAGCATTTGTTTCGAGTCTCTGACGACGGACTTGGAACACC CATCACCACCCGAAGCTTTTGCAAAGATCCGATTGGTCCTGGAAGTGCTCCAGAAACAGCTTCTAGATTT GCCTCTTGATGCTGACCTAGAAATTGGAAAAGTCCCTGGGATCGTGTTGGCTGAGATGCTTGGTGAGGGG ATCTGGGAGGACCTTTCTGAGTGCCTCATCAGAAACTGCTTGGTTTACTCTATTCCCACAAACAGCAGCA AGCTCCAGGAATACGAAGAGATTATACAGTCCACAGAAGAATTTGAAAAGTTCCTAAAGGAGATGAGGTT TTTAAAAGGCGATACTACTGACTTGCTGAAATATGCTCGCAATATCAATTCTCATTTTGCTAATAAGAAG TGCCAGGATGTGATTGTGGCGGCTAGAAATCTCATGACCTCTGAGATTCACAACACTGTCAAGATTGGTC CCGATTGTAAGGAAGCCTTACCAGACTTACCCAGTCCTGATGCTGATCACAAGCTACAAGTACAGACGGT CTGCAAAGCGCAGTTCACCGACGCCGGGAACCTAGAGCCTGAGACTTCTCTGGACCCTCAGTCCTTTTCT TTACCCACGTGCCGCATCAGCGAGGCTGTGAAGAAGTTAATGGAGCTCGCTTATCAGACTTTACTTGAGG CAACAACAAGTAGCGACCAATGTGCTGTCCAGCTCTTCTACTCAGTGAGGAATATCTTCCATTTGTTCCA TGATGTGGTACCAACATACCACAAGGAGAACCTTCGGAAGCTCCCCCAGTTGGCTGCCATTCACCACAAC AACTGCATGTATATTGCTCACCACTTGCTGACCCTCGGTCATCAGTTCAGATTGCGGCTCGCCCCCATCC TTTGCGATGGCACGACCACCTTTGTGGATCTTGTACCTGGCTTCAGAAGACTTGGGACGGAGTGCTTTTT GGCCCAAATGCAGGCACAGAAAGGAGAACTTCTGGAGAGATTGTCAAGTGCTAGAAGCTTTGCGAACATG GACGACGAAGAGAATTACTCTGCAGCGAGCAAGGCAGTGAGGCAGGTGCTGCACCAGCTAAGGAGACTTG GGATCGTGTGGCAGGATGTCCTGCCAGTGAACATATACTGCAAGGCCATGGGGACCTTGCTGAACACAGC GATTGCTGAGATGATGAGCAGGATCACTGCTCTAGAGGACATCTCGACTGAAGATGGTGATAGATTGTAT TCCCTGTGCAAAACAGTGATGGATGAAGGTCCACAAGTGTTTGCACCTCTGTCTGATGAAAACAAAAACA AGAAATACCAAGAAGAGGTTCCCGTCTATGTGTCCAAATGGATGCCTTTCAAAGAACTCATGATAATGCT ACAAGCTAGCTTGCAAGAAATTGGAGATCGGTGGGCAGATGGAAAAGGGCCCCTGGCAACTGCATTCCCT TCCAGCGAAGTCAAAGCTTTAATCCGTGCCTTGTTCCAGAACACAGAAAGAAGAGCAGCTGCCCTTGCTA AGATTAAATAGCTCCTCCTTGGAGAAAGGCTCTCCTGACTCTGGGTTCCTCCTTTGGTGTAATTATTTCC TTCAAAACCTCTCGGAATTACTTGTTGGTTTTGGTGAGCCAGTATATCATGGAAGCGTAACTTCACATAA GGACATCCTTGCCTGGACGAAGGTTTTCTGAACTGGACATTACGATGATCAATTTACGCAAAGCACTGGT GGGTTCATTGACTGTCGCTGCTGTCCCTTGTCAAGTATGAGTCTGCTCCGCAGTGTGATTTGTTCCCAGG CCCTCTTGAGAGTTGTATACATTATAATTTTAGACCCTGGCTTTGGACATTCCAAAGTTAGGGTTCAGAA TTTGTAAAATACGTTTTTTGAGGGGGGTGGGCATTAAGGGCACATTACTTGTTCTTACAAGAATTACAGA AACCTCTTAAAATATTTCTTTCCAGTAAATGCCTTTCAGAGTTGAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC012435 |
| Insert Size | 2340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012435, AAH12435 |
| RefSeq Size | 2866 bp |
| RefSeq ORF | 2340 bp |
| Locus ID | 26951 |
| Gene Summary | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).[UniProtKB/Swiss-Prot Function] |
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