Pcaf (BC082581) Mouse Untagged Clone
CAT＃: MC206206
Pcaf (untagged) - Mouse p300/CBP-associated factor (cDNA clone MGC:100182 IMAGE:30653109), (10ug)
CNY 5960.00
货期*
现货
规格
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A930006P13Rik; AI461839; AW536563; p/CAF; Pcaf |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC082581
GACAGTCGGGGCGGCATGGCCGAGGCTGGCGGGGCCGGGTCCCCAGCACTGCCTCCCGCGCCCCCGCACG GTTCCCCCCGGACCCTGGCCACCGCTGCCGGGAGCTCTGCTTCCTGCGGGCCAGCGACCGCGGTGGCCGC GGCGGGCACGGCCGAGGGACCGGGAGGAGGCGGCTCGGCCCGGATCGCCGTGAAGAAGGCGCAGTTGCGC TCTGCTCCGCGGGCGAAGAAGCTGGAGAAACTCGGCGTGTACTCCGCCTGCAAGGCAGAGGAGTCCTGTA AATGCAATGGCTGGAAGAACCCTAACCCCTCTCCTACTCCACCAAGAGGAGACCTCCAGCAGATAATTGT CAGTTTGACAGAATCCTGTCGAAGCTGTAGCCATGCCCTTGCTGCTCACGTTTCTCACTTGGAGAATGTG TCAGAGGAAGAGATGGACAGACTCCTGGGAATTGTGTTGGATGTGGAGTACCTCTTCACCTGCGTCCACA AAGAAGAAGATGCAGATACCAAACAAGTGTACTTCTACCTATTCAAGCTCTTGAGAAAGTCAATTTTACA AAGAGGAAAACCTGTGGTTGAAGGCTCCTTGGAGAAGAAGCCGCCATTTGAGAAGCCCAGTATTGAACAG GGTGTGAACAACTTCGTGCAGTACAAGTTTAGTCACTTGCCATCGAAAGAGAGGCAGACAACGATCGAGC TGGCCAAGATGTTTCTGAACCGCATCAACTACTGGCATCTGGAGGCTCCATCTCAGCGGAGACTACGGTC TCCCAATGATGACATCTCTGGATACAAGGAAAACTACACAAGGTGGTTGTGCTACTGCAATGTACCGCAG TTCTGTGACAGCTTACCTCGGTACGAAACCACAAAGGTGTTTGGGAGAACATTGCTTCGCTCGGTCTTCA CCATCATGAGACGACAGCTCTTGGAACAAGCCAGACAGGAAAAAGACAAACTGCCTCTTGAGAAACGCAC GCTTATCCTCACACATTTCCCAAAGTTTCTGTCCATGTTGGAAGAAGAAGTGTATAGTCAAAATTCTCCT ATCTGGGATCAGGATTTTCTCTCAGCCTCTTCCAGAACCAGCCCGCTAGGAATCCAAACAGTAATCAGTC CTCCTGTTACTGGGACAGCATTGTTCAGTTCAAATTCCACTTCCCATGAGCAGATCAATGGAGGGAGAAC AAGTCCTGGATGCAGAGGCTCTTCTGGGCTTGAAGCAAACCCGGGAGAAAAGAGGAAAATGAACAACTCT CATGCTCCCGAGGAGGCCAAGAGATCTCGAGTGATGGGGGATATTCCCGTGGAATTGATCAATGAGGTCA TGTCTACCATCACAGACCCTGCAGGGATGCTTGGACCAGAGACCAATTTTCTGTCAGCCCATTCGGCCAG AGATGAGGCGGCACGGCTGGAAGAACGCAGGGGTGTCATTGAATTCCACGTGGTGGGCAACTCCCTGAAC CAGAAACCAAACAAGAAGATCCTGATGTGGCTCGTGGGCCTCCAGAATGTGTTTTCCCACCAGCTGCCCA GAATGCCCAAAGAGTACATCACACGGCTCGTCTTTGACCCGAAACACAAAACCCTTGCTTTAATTAAAGA TGGCCGTGTCATTGGTGGTATCTGTTTCCGGATGTTTCCATCCCAGGGATTCACAGAGATTGTTTTCTGT GCAGTAACCTCAAATGAACAAGTCAAGGGCTATGGAACCCACCTGATGAACCATCTCAAAGAATACCACA TAAAGCACGAGATCCTCAACTTCCTCACATATGCAGATGAGTATGCCATCGGCTATTTCAAGAAGCAGGG TTTCTCCAAAGAAATCAAAATACCTAAAACCAAATATGTTGGCTACATCAAGGATTATGAAGGGGCCACT TTGATGGGATGTGAGCTGAACCCTCAGATCCCATACACAGAGTTCTCTGTCATCATTAAAAAGCAGAAGG AGATCATTAAAAAGCTGATAGAAAGAAAACAAGCCCAGATTCGAAAAGTCTACCCTGGACTTTCGTGTTT CAAAGATGGAGTTCGGCAGATTCCTATAGAAAGCATTCCTGGAATCAGAGAGACAGGCTGGAAACCAAGT GGAAAAGAGAAAAGTAAAGAGCCCAAAGACCCTGAGCAGCTTTACAGCACCCTCAAGAACATCCTGCAGC AGGTGAAGAACCATCCAAATGCTTGGCCTTTCATGGAACCAGTGAAGAGAACAGAAGCTCCGGGATATTA TGAAGTTATAAGGTTCCCCATGGATCTGAAAACCATGAGTGAACGCCTCAGGAACAGGTACTATGTGTCT AAGAAGTTATTCATGGCGGACTTGCAACGAGTGTTCACCAACTGCAAGGAGTACAACCCTCCCGAGAGCG AGTACTACAAATGCGCCAGCATCCTGGAGAAGTTCTTCTTCAGTAAAATTAAGGAAGCAGGGTTGATTGA CAAGTGATTTCTTTCCCTTAGACACACATCAGCAGTGTGCCTAAAACAGGTCATTTAGGTTTTTGGTTGC TTTTGTTTTTTTGTTTTTGTTTTTAAATTGTACATCATGTGTTGAAGAGACTTGTAATAATTAGCACTTT TGAAAAACAAACAAAACCTCCTTTTAGCTTTTCAGAGACGTATTTAAATGGAAATCATAGATTTTTTTTT TTATTTTGAGCAATAGATTTTAATCTATTTACTACTATCATGTAATTTTTCTCTGGCATGTCCAGTAGCT GACTATTGCATGGTAGATGATCAGGGGCTTCCTCAAAACCTGTATGTGAGGAAATTGCACACAGTACCAA GATTTGGGGTAATCTAGAGAAAAGTTTGACCATGAATGTCTCCATTTTTTTTCTAATGGAACATGAGAGT TTATTTTATTCTGAAGGACTTTAAAGGGTCCGTGACACACCTGTGCTGTGAATGAACTGTGTGATGTTTG AAGTCTCCTTGTAGACTTTTTATAAGTATATATTTTTAAAACACTCATCTAGATGAGGTGCTTTGAGCAG TTCTGAAAAAAAAAAAATGCAGCTTCCACAATAGCGGCTGCTATGACTCCTAAGGAAGGGACTTTTTATA AGCTCCTGGGGCTTTGATCATTCTGTCTACCTATTACAGACTTGAGAGTGCACAACCACTATTTAATAAT ATCTTGGCAGATATGTGTCCATCTCATATTTGACTTTGCTTGTGCAGGGCCCAACTTCTAAAAGATCCGT TCCCAGGCCACAAAGGTGTTAGCTTGAAAATATTTGAGGTTGAGTTAATGTTAAGACATGTGGCCTAAAC ATGAACCCAGGGGAGCCTTGCTTCACTATGTAAGGATTCTTTGTATTTTCTGGTACATTTAACCCCATCA CCAGTGTCTATGTGAACATTTCCCATTTGCACTTCCAGAGTTTTGTTTGTCTGAGGTGAACTGAATTCTC ACCAAAGTTTAGGCCAGTATTTTAACACCTCCATTGTTTTATTGGTCCAGGGTTATATCCCACTAACATT CGCTGTACAATCTTATTAACCATTTTTGTCTTGGGACTCCAGCTTAGTGCTTGGATTTATTTCCTAATTA CACTACATAGAAAAGTGGGACACCTGCCAGCCCAACTCTGGGAAAAACCAACTAATATACAACCATATTA AATGAAGGCCACCTTAATGGTCTCGACACTAATTTTTATGATGCAAATTTATAAACTGATTTTTTTGTAA AGGCTGAGGTTTTAAAAATGTATTTAATGTATGTTTTCTATCCGCATAAATTATATTGGGTCACTAATAG TCATTAAAAATAGCTTGCAGAGGTAGTGTGCTTGAAGGAAAATATCCTGGCATTGCTCCCGAGTGCTGCT TCTGACATGGAAGGCATGCTGTATGTTCATAGTGTTCACTGAAGATCACACTGAAGATGCCTCAACATTG GGAAATGTAAGGAACACTTTGTGTGGTATCTAGACTATTAATGAAGAGTGATAATAGAGGCTGCATTAAG TGTCTCAGACTGGTTTTAAAACAGCTTTGTACCGATTTCGCTGAAGCGTCTGTCCAGTTTGGTATCCTGT GAAAGTTTGTTATTTCCTGGGTAGACATCCTTAAAGAGTATTGTCTTTAAAATCAGATGGTCTCTTCTAT ATTGAAAGCATTTTTATGTTTTCTAATTTAAAATTAATATTTTCTTATAGATATTGTGCAATAAAGCAGA AGTAGAATGTGGTTTTTGCAAATGCTTTAACGGCCGACAAAAACTTTACATTTGTAAAATTAATATATTG TACTGGTACAAAATAGTTTTAAATTATATTTTGAAAAGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC082581 |
| Insert Size | 2442 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC082581, AAH82581 |
| RefSeq Size | 4344 bp |
| RefSeq ORF | 2442 bp |
| Locus ID | 18519 |
| Gene Summary | Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Also acetylates spermidine (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PCAF interacts with XBP-1S and mediates XBP-1S-dependent transcription
,null,
Nucleic Acids Research
,PubMed ID 20817929
[Pcaf]
|
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