Kcnj9 (BC065161) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Girk3, Kir3.3, mbGIRK3
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC065161 CTCAGGGGGGAAGAATTTGAAGCAAAACCAGACCCCGCAGGATCCCCGCTGCGGCCGCCATGGCGCAGGA GAACGCCGCTTTCTCTCCCGGGTCGGAGGAGCCGCCACGCCGCCGCGGTCGCCAGCGCTACGTGGAGAAG GACGGTCGCTGTAACGTGCAGCAGGGCAACGTCCGCGAGACCTACCGCTACCTGACCGACCTGTTCACCA CGCTGGTGGACCTGCAGTGGCGCCTCAGCCTGCTCTTCTTCGTGCTCGCCTACGCGCTCACTTGGCTCTT CTTCGGCGCCATCTGGTGGCTCATCGCCTACGGCCGCGGCGACCTGGAACACCTGGAGGACACCGCGTGG ACCCCGTGCGTCAACAACCTCAACGGCTTCGTGGCCGCCTTCCTCTTCTCCATCGAGACGGAGACCACCA TCGGCTATGGGCACCGGGTCATCACCGACCAGTGTCCCGAGGGCATCGTGCTGCTGCTGCTGCAGGCTAT CCTGGGCTCCATGGTGAACGCTTTCATGGTGGGCTGCATGTTCGTCAAGATCTCGCAGCCCAACAAGCGC GCCGCCACTCTCGTCTTCTCCTCGCACGCCGTGGTGTCTCTGCGCGACGGGCGCCTCTGTCTCATGTTTC GCGTGGGCGACCTGCGATCCTCGCACATCGTCGAGGCCTCCATCCGCGCCAAGCTCATCCGCTCCCGTCA GACGCTCGAGGGCGAGTTCATCCCTTTGCACCAGACCGACCTCAGCGTGGGCTTTGACACGGGGGACGAC CGCCTCTTTCTCGTCTCACCTCTCGTCATCAGCCACGAAATCGATGCCGCCAGCCCCTTCTGGGAGGCAT CGCGCCGCGCCCTCGAGAGGGACGACTTCGAGATCGTAGTCATTCTCGAGGGCATGGTGGAGGCCACGGG AATGACGTGCCAAGCTCGAAGCTCGTACCTGGTGGATGAAGTGTTGTGGGGCCACCGGTTCACATCGGTG CTCACCCTGGAGGATGGTTTCTATGAGGTGGACTACGCCAGCTTCCACGAAACCTTTGAGGTGCCCACAC CCTCGTGCAGTGCTCGGGAACTGGCAGAAGCCGCGGCCCGCCTCGATGCCCATCTCTACTGGTCCATCCC CAGCAGGCTGGATGAGAAGGTGGAGGAAGAAGGGGCTGGGGAGGGGGCAGGTGCGGGAGATGGAGCTGAC AAGGAGCACAATGGCTGCCTGCCACCCCCAGAGAGTGAGTCCAAGGTGTGACTGGTTTCCTCCCACCCCC TGTGGCAGACCAGGGGGCCGGACTCAGGTACACAGAAGCTGCGAGTGGAGGTGGAAGAAGAGGAGGCAGG CAGTGTCCCGAGGAACAGCTAAAGTTGGGAGAGGCCCGCTGAGTCCAGGATCGAGTAGGGAAGGCTGAGG TCCTGGTTTGAAGAGAGAGGGTTGCAGGGCGGGGTGAGAGAACGTGTCAGTCTGTCTGTGTTTGACCTTC ACATCGGTTCATGGGTGGATGGATGGACAGAAGGATGGGCTCATGGGGGTTGATCGGGAAGGTGGGGCAG ATAGAGACAGCCAATGGATAGCTCAGGTGGTAAGTGGCTTGACAGTCGATGATTGTCACCTGCAGCACAC CTTTGTGAGAAATCCATGGGCATCCTTTTCTTCCAGATATAGGTAGCCTCAAACCAGGGAGCGTGGCTTA GGGAGCAGGCTGTCAGGTGGACTACCATCCCCACTCACCTCCCCTCAACTGGCCTCCCTATGTGTGACAC GCCTGCCTAACTAGAGAAGAGAGCACTGGGTAGAGGTGGGCACAGGTGTGGGTGCCCTCCCCAGCATCAC TGTCCCATGGCGAGAGGTCAGAAAGGCAAACAAGCAATGGGGGTAGATGCTGAGCAGGGAGGGGCCCTGA AGCAGGACCTGGGGACAGCCAAGGACAACTATTTTGTGAGAGAGGAATGAAACCTTGCAGGTCCTGCCAC AGAAGCAAGAAGCAGAGGAAAGGCCATGGAGAGACTTAATAAAGGGTTTTACAAGGGTACCTGGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC065161
Insert Size	1182 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC065161, AAH65161
RefSeq Size	2060 bp
RefSeq ORF	1182 bp
Locus ID	16524
Gene Summary	This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]

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