Spdl1 (NM_027411) Mouse Untagged Clone
CAT＃: MC205646
Spdl1 (untagged) - Mouse coiled-coil domain containing 99 (Ccdc99), (10ug)
CNY 4536.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700018I02Rik; 2600001J17Rik; 2810049B11Rik; AA409762; Ccdc99 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC006674
GCTTACTTAGCAGTGCTGCTGCCGAGTGAAGAGGGAGTTATTTCTGTGCTGAGGTGGAGTTAGTGTCACG AATCTGGGAGTCCAGTGCTCTCCGGACAGGCCTCCGGTTGCAGCAGCCAGGAAGCGTGAGGAGAGAGTAG GTTGAGAACAGACATCATGGAGGCAGATATCACAAATCTTCGGAACAAACTCAAAGAGTGTGAAGACGAG CGACTGAAAGCTGCACACTATGGCCTACAGCTCTTGGAGAGGCAGACGGAACTGCAGAGCCAACTGGATA AATGTCATGAAGAAATGATGATCACAGCAGAGAAATACAACCAAGAGAAGCATGCCCTTCAAAGAGAAGT GGAGCTCAAGAGTCGGATGTTAGACAGCCTGAGCTGTGAGTGTGAAGCTCTGAAACAGCAGCAGAAGGCG CAGCTGGAGCAGCTAGAGGTGCAGCTACATAGGAGCCACCGGCAGGAAGTGAGCGACCTGAAGAACAAGT TAGAAAATCTGAAAGTGGAATTGGATGAAGCAAGGCTTGGCGAGAAGCAGCTGAAGCAGAAGCTGGATCT GCAGGGGGAACTTCTCGCTCACAAGTCAGAAGAGCTCCGGCTCCTGTCTGAGCAGAGAGTGCTCAGTAGC ATGTCTTCGGAGCTGCTGGCTCTTCAGACTGAGCTGACGGCAGCGGAAGGTGTGAAGAATGCCCTCAAAG AAGAGGTGAATGAACTGCAGTACAAACAAGAGCAGCTAGAATGTCTCAATACTTCCTTACTGCACCAGGT AGACAGGCTTAAAGAAGAAAAAGAGGAGCGTGAGAGAGAAGCGGTCTCTTACTATAATGCCTTGGAGAAA GCTCGTGTAGAGAACCAGGATCTTCAGGTACAGCTGGGTCACGCACTCCAGCAGGCGGCGGACCCCAATA GCAAAGGGAACTCGCTGTTCGCAGAGGTGGAGGATCGAAGGGTGGCAATGGAACGCCAGCTCAACTTGAT GAAAGACAAATACCAGTCACTGAAGAAGCAAAATGCGTTCACCAGAGATCAGATGAACAAGATGAAGTTA CAAATCTCCACATTGCTGAGGATGAGGGGCTCGCAGACTGAGTTTGAACAGCAGGAGCGGTTGTTTGCCA TGATAGAACAGAAGAATGGTGAAATAAAACACCTTTTAGGGGAAATTAATAAACTGGAGAAATTTAAGAA CTTATATGAAAGTATGGAATCGAGGCCTTCCACATCAGACACCGCTTGTGTGCTAGAAGACAGCACCTAT TACTCAGATCTACTTCAGCTGAAGCTTGATAAGCTAAACAAAGAAAATGAAAGCACAAAGGACGAGCTGT CCATCCAGAGGATGAAGGCTTTATTTGAGAGCCAGCGGGCCCTGGATATTGAGCGGAAACTGTTTACAAA TGAAAGACACCTGCAGCTCTCAGAAAGTGAAAACATGAAACTGAGAGCGAAGCTAGATGAACTCAAGCTG AAGTATGAACCGGAAGAGAGAATTGAAGTACCTGTACTCAAAAGGAGGCGCGAGGTGCTGCCATTGAATA TAACCACGCCAGAAGAAACAGAAGAAACAGCTGCTGCCAGTGCCACTGAGGATGGAGTTTCTAGACTCCC ACCTCACAGGGAGGAGGAATCCTGTCTTAATAGCTTAAAAGATAACACTGTGCAGTGGAAACAGCCAGCC TCTTCATGTGTGCAGCCAGCCAGCCTCTCTCCTCATAAGAACCTGCATCTGGATACACAGCCGAAGAAGG AGAAGAAGTGTGTGAAGCTTGTGGATAGTCCGGCCAACATTGAGGTCTTACATGAACAAAGTGGGAATAC CCCCAATTCTCCCAGGTTAACTGCAGAATCAAAGCTTCCAACAGAAGTGAAGGAAAGGATAGAAACTACA AGCAAATTGGGAAAAGGAGCTTGTAAGAAATCACACAACATCATATATGTGTCCTCAAAGTCAGCCCCAG AAACGCAGTGCTCACAGCAGTAGTGAGGCCTGCGCGCTGGGCCACGCTAACCATCGCTCCTATGCGTGGG ACACCCTTGTGGAGCTTTGCATAGGTCAGTGCTGAAGCCTGGCGTGCTCGCACCGCTGTGACCAAGACTT CCGATGTGGCTGTGATTCTGACCCATAAGTCCTTAACTGATGATCCTTCTCAGAACTGTGGGTCACAGGG AACAGACCAGATCAGAAGAGAATGGGAGAATTTAAACCTACTTCCTGTGTTGGATGTGGAGTTTGAACAT GGTTAAATAATCTGTCATAAGAACCAAAAATTAGAAAATGGAACACTTAAAATTATTTATAATTTTCTTT GTACATCGTTTTGCACTTAGATTTTCCTTGTCTTGTAAAAATAATGAAATTGGCTTGAAAATATATATTA CTACAAAATGTTTTAAAGTGTGAAGTACCTATATTCATATGTGTAATTTAGATTTTATGACTCAAACATC AATAATTTTGAAATTTAAAATAAAATTGCAGTAATTTCAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_027411 |
| Insert Size | 1827 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006674, AAH06674 |
| RefSeq Size | 2508 bp |
| RefSeq ORF | 1827 bp |
| Locus ID | 70385 |
| UniProt ID | Q923A2 |
| Gene Summary | Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209383 | Spdl1 (tGFP-tagged) - Mouse coiled-coil domain containing 99 (Ccdc99) |
CNY 5040.00 |
|
| MR209383 | Spdl1 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 99 (Ccdc99) |
CNY 4536.00 |
|
| MR209383L3 | Lenti ORF clone of Spdl1 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 99 (Ccdc99) |
CNY 6460.00 |
|
| MR209383L4 | Lenti ORF clone of Spdl1 (mGFP-tagged) - Mouse coiled-coil domain containing 99 (Ccdc99) |
CNY 6460.00 |
