Srebf1 (NM_011480) Mouse Untagged Clone

CAT#: MC205184

Srebf1 (untagged) - Mouse sterol regulatory element binding transcription factor 1 (Srebf1), (10ug)



  "NM_011480" in other vectors (3)

CNY 7,904.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-SREBF1 Antibody
    • 50 ug

CNY 5,200.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms ADD-; ADD1; bHLHd; bHLHd1; SRE; SREB; SREBP; SREBP1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC056922
CCTTAACGTGGGCCTAGTCCGAAGCCGGGTGGGCGCCGGCGCCATGGACGAGCTGGCCTTCGGTGAGGCG GCTCTGGAACAGACACTGGCCGAGATGTGCGAACTGGACACAGCGGTTTTGAACGACATCGAAGACATGC TCCAGCTCATCAACAACCAAGACAGTGACTTCCCGGGCCTGTTTGACGCCCCCTATGCTGGGGGTGAGAC AGGGGACACAGGCCCCAGCAGCCCAGGTGCCAACTCTCCTGAGAGCTTCTCTTCTGCTTCTCTGGCCTCC TCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTGCACCCTTGTCCCCTCCACCATCGGCACCCG CTGCTTTAAAGATGTACCCGTCCGTGTCCCCCTTTTCCCCTGGGCCTGGGATCAAAGAGGAGCCAGTGCC ACTCACCATCCTACAGCCTGCAGCGCCACAGCCGTCACCGGGGACCCTCCTGCCTCCGAGCTTCCCCGCA CCACCCGTACAGCTCAGCCCTGCGCCCGTGCTGGGTTACTCGAGCCTGCCTTCAGGCTTCTCAGGGACCC TTCCAGGAAACACTCAGCAGCCACCATCTAGCCTGCCGCTGGCCCCTGCACCAGGAGTCTTGCCCACCCC TGCCCTGCACACCCAGGTCCAAAGCTTGGCCTCCCAGCAGCCGCTGCCAGCCTCAGCAGCCCCTAGAACA AACACTGTGACCTCACAGGTCCAGCAGGTCCCAGTTGTATTGCAGCCACACTTCATCAAGGCAGACTCAC TGCTGCTGACAGCTGTGAAGACAGATGCAGGAGCCACCGTGAAGACTGCAGGCATCAGCACCCTGGCTCC TGGCACAGCCGTGCAGGCAGGTCCCCTGCAGACCCTGGTGAGTGGAGGGACCATCTTGGCCACAGTACCT TTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTCGCAGCTGGCAGCAAGGCCCTAGGCTCAGCTC AGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAAGCGCTACCGGTCTTCTATCAATGACAA GATTGTGGAGCTCAAAGACCTGGTGGTGGGCACTGAAGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAG GCCATCGACTACATCCGCTTCTTGCAGCACAGCAACCAGAAGCTCAAGCAGGAGAACCTGACCCTACGAA GTGCACACAAAAGCAAATCACTGAAGGACCTGGTGTCAGCTTGTGGCAGTGGAGGAGGCACAGATGTGTC TATGGAGGGCATGAAACCCGAAGTGGTGGAGACGCTTACCCCTCCACCCTCAGACGCCGGCTCACCCTCC CAGAGTAGCCCCTTGTCTTTTGGCAGCAGAGCTAGCAGCAGTGGTGGTAGTGACTCTGAGCCCGACAGTC CAGCCTTTGAGGATAGCCAGGTCAAAGCCCAGCGGCTGCCTTCACACAGCCGAGGCATGCTGGACCGCTC CCGCCTGGCCCTGTGTGTACTGGCCTTTCTGTGTCTGACCTGCAATCCTTTGGCCTCGCTTTTCGGCTGG GGCATTCTCACTCCCTCTGATGCTACGGGTACACACCGTAGTTCTGGGCGCAGCATGCTGGAGGCAGAGA GCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCTAGTCTGGCTGGCCAATGGACTACTAGT GTTGGCCTGCTTGGCTCTTCTCTTTGTCTATGGGGAACCTGTGACTAGGCCACACTCTGGCCCGGCTGTA CACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCCGGGGAGATTTCCCCCAGGCTGCTCAAC AGCTGTGGCTGGCCCTGCAAGCGCTGGGCCGGCCCCTGCCCACCTCAAACCTGGATCTGGCCTGCAGTCT GCTTTGGAACCTCATCCGCCACCTGCTCCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGGCCAGGCCGGG GGCCTGCTGAGGGACCGTGGGCTGAGGAAGGATGCCCGTGCCAGTGCCCGGGATGCGGCTGTTGTCTACC ATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGGACATCTTGCTGCTTCTAACCTGGCACT AAGTGCCCTCAACCTGGCTGAGTGCGCAGGAGATGCTATCTCCATGGCAACACTGGCAGAGATCTATGTG GCAGCGGCCCTGAGGGTCAAAACCAGCCTCCCAAGAGCCCTGCACTTCTTGACACGTTTCTTCCTGAGCA GCGCCCGCCAGGCCTGCCTAGCACAGAGCGGCTCGGTGCCTCTTGCCATGCAGTGGCTCTGCCACCCTGT AGGTCACCGTTTCTTTGTGGACGGGGACTGGGCCGTGCACGGTGCCCCCCCGGAGAGCCTGTACAGCGTG GCTGGGAACCCAGTGGATCCGCTGGCCCAGGTGACCCGGCTATTCCGTGAACATCTCCTAGAGCGAGCGT TGAACTGTATTGCTCAGCCCAGCCCAGGGGCAGCTGACGGAGACAGGGAGTTCTCAGATGCCCTTGGATA TCTGCAGTTGCTAAATAGCTGTTCTGATGCTGCCGGGGCTCCTGCTTGCAGTTTCTCTGTCAGCTCCAGC ATGGCTGCCACCACTGGCCCAGACCCAGTGGCCAAGTGGTGGGCCTCACTGACAGCTGTGGTGATCCACT GGCTGAGGCGGGATGAAGAGGCAGCTGAGCGCTTGTACCCACTGGTAGAGCATATCCCCCAGGTGCTGCA GGACACTGAGAGACCCCTGCCCAGGGCAGCTCTGTACTCCTTCAAGGCTGCCCGGGCTCTGCTGGACCAC AGAAAGGTGGAATCTAGCCCAGCCAGCCTGGCCATCTGTGAGAAGGCCAGTGGGTACCTGCGGGACAGCT TAGCCTCTACACCAACTGGCAGTTCCATTGACAAGGCCATGCAGCTGCTCCTGTGTGATCTACTTCTTGT GGCCCGTACCAGTCTGTGGCAGCGGCAGCAGTCACCAGCTTCAGTCCAGGTAGCTCACGGTACCAGCAAT GGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATGACCTGAGCAGCCTGCGGCGGTTGGCAC AGAGCTTCCGGCCTGCTATGAGGAGGGTATTCCTACATGAGGCCACAGCTCGGCTGATGGCAGGAGCAAG TCCTGCCCGGACACACCAGCTCCTGGATCGCAGTCTGAGGAGGAGGGCAGGTTCCAGTGGCAAAGGAGGC ACTACAGCTGAGCTGGAGCCACGGCCCACATGGCGGGAGCACACCGAGGCCCTGCTGTTGGCATCCTGCT ATCTGCCCCCTGCCTTCCTGTCGGCTCCTGGGCAGCGAATGAGCATGCTGGCCGAGGCGGCACGCACCGT AGAGAAGCTTGGCGATCACCGGCTACTGCTGGACTGCCAGCAGATGCTCCTGCGCCTGGGCGGCGGAACC ACCGTCACTTCCAGCTAGACCCCAAAGCTTTCCCTTGAGGACCTTTGTCATTGGCTGTGGTCTTCCAGAG GGTGAGCCTGACAAGCAATCAGGACCATGCCGACCTCTAGTGGCAGATCTGGAAATTGCAGAGGCTGCAC TGGCCCGATGGCACCCTCTTGCTCTGTAGGCACCTTAGTGGCTTTTCCCTAGCTGAGGCTCACCCTGGGA GACCTGTACATAGTGTAGATCCGGCTGGGCCTGGCTCCAGGGCAGGCCCATGTACTACTTTGACTTTTGC AAACTTTATTTTCATAGGTTGAGAAATTTTGTACAGAATATTAAAAAATGAAATTATTTATAACTTCAGT TTTGTTTATCTGGGTAGGCAGATGTCTGCCTCAGCTGATTGCTATCTTTCCTGGCTTGTCCTTTGGGAAG CCGGACTTCCCATCTGTTGTAAGGTGTATTTGCTGGCTTGGTGATGCTATGTTGAGCCACCTTGGCCCTC TGATCTCATGGCTCATAACCCTTGAGGGGCCTTGATGGTCTCAGTTGTTCCTTTGCCTTCCAGCCCTCCA CCAGGTAATAACCCCGTATATCCAGAAACACACACAGTTCCCTAGTCATCTCTGTGCACCAGCATAGGCG AAGGATCAGGGCCCATTAGTGAGTAGACTGGGAACCCTGTTTGTGTCCCCCGGCCCCTTCCCTCTACTCC CAACCCCTTAGGGAATCTGGTCGGCATCCACAGGCCGCACACTTCCAAAACAATCGTGGTATCTTTATTG ACTTTTTTTTTTCTGAATGCAATGACTGTTTTTTTTTTTTTTAACTCTTAAGGAAAATAAACATCTTTTA GAAACAGCTCGATACAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN NM_011480
Insert Size 3405 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC056922, AAH56922
RefSeq Size 4306 bp
RefSeq ORF 3405 bp
Locus ID 20787
UniProt ID Q9WTN3
Gene Summary This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Alternatively spliced transcript variants have been characterized for this gene. [provided by RefSeq, Nov 2017]
Transcript Variant: This variant (1, also known as SREBP-1a) represents the longest transcript and encodes the longest isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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