Gfm1 (NM_138591) Mouse Untagged Clone
CAT＃: MC204542
Gfm1 (untagged) - Mouse G elongation factor, mitochondrial 1 (Gfm1), nuclear gene encoding mitochondrial protein, (10ug)
CNY 5512.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW545374; D3Wsu133e; Gfm |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC031772
GACTTTGAGCTCTTCCCGCTCTGCTGCCGGCGCTGAGCACAACCGATCACCTCAGAGCAGGAGCCTTGGA AGTGGCTGTCCCAGGCTTGCCATGCGGCTGCTGCGGGTCGCCGCAGCCCTGGGGCGTGGACCGTTCCCTC GGGTCCCTGCAGTCCTGGGCTGGCAGGGGAAGCAGGCTGATTGGAAGACCCGCCGATGGTGTTCGTCAGG GCCAGTTCCCAATGAAAAAATACGAAACATTGGGATCTCAGCTCACATTGATTCTGGGAAGACGACGCTA ACAGAACGGGTGCTATACTACACTGGCAGGATTGCCACGATGCACGAGGTGAAAGGTAAAGATGGAGTTG GCGCTGTCATGGATTCCATGGAGCTGGAGAGGCAGCGAGGGATCACGATCCAGTCAGCAGCTACGTATAC CATGTGGAAAGACATCAATATCAACATCATAGACACGCCAGGCCACGTGGACTTTACCATAGAAGTGGAA AGAGCCCTGCGGGTCCTGGACGGTGCAGTCCTTGTTCTCTGTGCGGTTGGCGGAGTCCAGTGCCAGACCA TGACAGTCAGTCGTCAGATGAAGCGCTACAACGTTCCCTTTTTAACCTTCATTAACAAACTGGACCGAAT GGGCTCTAACCCATCCAGGGCCCTCCAACAAATGAGATCTAAACTAAATCACAATGCTGCATTTGTACAA ATACCCATTGGTTTAGAGGGTGATTTTAAGGGAATTATAGACCTCATTGAAGAACGAGCTATTTACTTTG ATGGAGACTTTGGTCAGATTGTCCGATATGACGAGATTCCGGCTGGATTGCGGGCCGCAGCCGCTGACCA CCGGCAGGAGCTCATTGAGTGCGTTGCTAACTCAGATGAGCAGCTTGGTGAGCTCTTCCTGGAAGAAAAG ATCCCCTCAGTTTCCGACTTAAAGCGAGCAATCCGGAGAGCTACTCTGAGTCGATCCTTCACTCCTGTAT TTTTGGGAAGTGCTTTGAAAAACAAAGGAGTTCAACCTCTTTTGGACGCTGTTTTAGAATACCTGCCCAA TCCATCTGAGGTCCAGAATTACGCTATACTCAATCAGAATGACTCAAAAGAGAAGACCAAAATCTTAATG AACCCCCAAAGAGATGATTCGCACCCATTTGTAGGCCTGGCTTTTAAACTGGAGGCGGGCCGTTTTGGAC AGCTGACTTATGTCCGAAATTATCAAGGTGAACTGAAGAAGGGAAGCACCATCTACAACACGAGGACCGG GAAGAAAGTGCGCGTGCAGCGGCTAGTGCGCATGCACGCCGACATGATGGAGGATGTTGAAGAAGTATAT GCTGGAGACATCTGTGCATTGTTTGGCATTGACTGTGCAAGTGGAGATACATTCACAAACAAAGATAACA GTGACCTTTCTATGGAATCGATTCATGTTCCTGAGCCTGTCATTTCAATAGCAATGAGGCCTTCTAACAA GAATGATCTGGAGAAATTTTCCAAAGGTATTGGCAGGTTTACACGGGAAGACCCCACATTTAAAGTCCAC TTTGACCCTGAGAGCAAGGAGACCATTGTGTCAGGGATGGGAGAGCTGCACCTGGAAATCTATGCTCAGA GAATGGAGAGAGAATATGGCTGCCCTTGTATCACAGGAAAACCCAAAGTAGCCTTTAGAGAGACCATTGT TGCCCCAGTGCCGTTTGATTTTACACATAAAAAGCAGTCGGGTGGTGCCGGCCAGTTTGGAAAAGTAATA GGAGTGCTGGAGCCCTTGCCCCCAGAGGACTACACGAAGTTGGAGTTTTCTGATGAGACATTTGGGTCCA ATGTTCCAAAGCAGTTTGTCCCCGCTGTTGAAAAGGGGTTTCTGGATGCCTGCGAAAAAGGCCCTCTTTC TGGTCACAAGCTTTCTGGGCTACGGTTTGTTCTGCAAGATGGAGCACACCACATGGTTGATTCGAATGAA ATCTCTTTCATCCGAGCTGGAGAAGGTGCTCTTAAACAAGCCTTGGCAAATGGCACACTATGTATTATTG AGCCTATTATGTCTGTGGAAGTTATAGCCCCCAATGAATTCCAGGGAACAGTGTTCGCAGGGATTAACCG GCGGCATGGAGTGATTACAGGGCAGGACGGCATTGAGGACTACTTCACACTGTATGCAGATGTTCCCCTA AATAATATGTTTGGTTATTCCACTGAGCTTAGGTCTTGCACAGAGGGAAAAGGAGAGTACACGATGGAGT ACTGCAGGTACCAGCCGTGTTCACCATCCACACAGGAAGAGCTCATCAATAAGTATTTGGAAGCTACAGG TCAGCTTCCCGTAAAAAAAGGGAAAGCCAAGAACTGACTCTCCTCACTCAGGATATACTGACTATTTGAC TACCACACTTGGGGATGATGGAGTCTGCTTGAATGAATTCTGACGGCAGAAACAAATTTAGGAGCCCCTG CTGGCCCACGTTCAGGAACTTCTGTATGTACGCCAAGGATAACTGTGTATTTAAACTGTATTGACTATTT TTATTGTTTAGTAAAAGACCTTAAATAAAATTATATTATTACTGAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_138591 |
| Insert Size | 2256 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031772, AAH31772 |
| RefSeq Size | 2578 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 28030 |
| UniProt ID | Q8K0D5 |
| Gene Summary | Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210468 | Gfm1 (tGFP-tagged) - Mouse G elongation factor, mitochondrial 1 (Gfm1) |
CNY 6370.00 |
|
| MR210468 | Gfm1 (Myc-DDK-tagged) - Mouse G elongation factor, mitochondrial 1 (Gfm1), nuclear gene encoding mitochondrial protein |
CNY 5512.00 |
|
| MR210468L3 | Lenti ORF clone of Gfm1 (Myc-DDK-tagged) - Mouse G elongation factor, mitochondrial 1 (Gfm1), nuclear gene encoding mitochondrial protein |
CNY 7700.00 |
|
| MR210468L4 | Lenti ORF clone of Gfm1 (mGFP-tagged) - Mouse G elongation factor, mitochondrial 1 (Gfm1), nuclear gene encoding mitochondrial protein |
CNY 7700.00 |
