Klhl20 (NM_001039482) Mouse Untagged Clone
CAT＃: MC203927
Klhl20 (untagged) - Mouse kelch-like 20 (Drosophila) (Klhl20), (10ug)
CNY 4504.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI504637; D930050H05Rik; Kleip; mKIAA4210 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC019571
ATCCTGCCTGCGCTAGCGGCTCCAGAGGTGGAGGCAGAGAGGGCGGAGAAGGCAGAGAGGGTGCCGCAGA GAGAAAAGTGTCAAAGCCAGTACGGCTTTAGAACACGGTGAAGGATACTTTTCATGGTGCAGGGAAGGAA AGCCAATGCGCAGGTGTACCAACATTCGACCAGGAGAGACTGGAATGGATGTAACAAGCCGCTGCACCCT TGGAGACCCCAACAAGCTGCCAGAAGGGGTTCCCCAACCTGCTCGCATGCCCTATATTTCAGACAAGCAC CCTCGGCAAACCTTGGAAGTGATCAACCTTCTGAGAAAGCACCGGGAGCTATGTGATGTGGTGCTAGTTG TGGGCGCTAAGAAGATATATGCCCACCGCGTCATCCTGTCAGCCTGCAGCCCCTACTTCCGAGCAATGTT TACAGGGGAGCTGGCAGAAAGCCGGCAGACAGAAGTAGTGATCCGAGACATAGATGAGAGGGCCATGGAG CTCCTGATTGACTTTGCATATACCTCCCAGATCACTGTGGAAGAGGGCAATGTTCAGACTCTCTTGCCTG CTGCTTGCCTCCTCCAGCTGGCAGAAATACAGGAAGCCTGCTGTGAATTCTTAAAGAGACAATTAGATCC TTCTAATTGTCTGGGCATTCGGGCTTTTGCTGACACACATTCTTGCCGTGAGTTGCTAAGGATTGCAGAC AAGTTCACCCAACATAACTTTCAAGAGGTAATGGAGAGTGAAGAATTCATGTTACTTCCGGCCAACCAAC TCATTGACATAATATCTAGTGATGAGCTCAATGTTCGGAGTGAAGAACAAGTGTTCAATGCAGTGATGGC CTGGGTCAAATACAGTATTCAGGAAAGACGGCCTCAGTTACCCCAGGTGCTGCAACATGTTCGCCTGCCT TTGCTTAGTCCCAAGTTCCTGGTGGGCACCGTAGGCTCTGACCCCCTCATTAAAAGTGATGAAGAATGCA GAGACTTGGTAGATGAGGCTAAAAACTACCTCCTGTTGCCTCAAGAACGACCACTAATGCAAGGGCCAAG GACAAGACCACGGAAACCTATCCGATGCGGAGAAGTACTCTTTGCAGTTGGTGGTTGGTGCAGTGGAGAT GCCATTTCCAGTGTTGAACGCTATGACCCCCAGACCAATGAGTGGCGAATGGTAGCTTCAATGAGCAAAA GGCGATGTGGAGTCGGGGTCAGTGTTCTTGATGACCTGTTATATGCAGTAGGCGGCCATGATGGATCCTC ATATCTCAATAGTGTTGAAAGATATGACCCCAAAACTAACCAATGGAGCAGCGATGTGGCCCCTACAAGC ACCTGCAGGACAAGCGTTGGTGTGGCAGTCCTTGGAGGTTTTCTCTATGCTGTGGGTGGACAGGATGGTG TCTCTTGCCTCAATATAGTTGAGAGGTATGATCCAAAGGAGAACAAGTGGACCCGGGTGGCTTCAATGAG CACTAGAAGGCTAGGGGTGGCTGTCGCCGTGTTGGGAGGGTTCTTATACGCTGTTGGTGGCTCAGATGGA ACATCTCCACTTAACACAGTGGAGCGCTACAATCCTCAGGAAAACAGATGGCACACCATAGCGCCTATGG GGACCCGAAGAAAACACCTAGGCTGTGCGGTGTACCAGGACATGATCTATGCAGTGGGAGGGAGAGACGA CACTACAGAGCTTAGCAGTGCTGAGAGATACAACCCCAGAACTAACCAGTGGTCTCCCGTGGTGGCCATG ACATCCCGCAGGAGTGGAGTCGGCCTGGCCGTGGTCAATGGACAGCTTATGGCAGTGGGGGGCTTTGATG GCACAACATACTTGAAGACCATTGAAGTTTTTGACCCAGATGCCAATACGTGGAGGTTATACGGAGGGAT GAATTACCGTCGGCTAGGAGGTGGTGTAGGAGTTATTAAAATGACACATTGTGAATCCCATATATGGTGA ACACAGGAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_001039482 |
| Insert Size | 1815 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019571, AAH19571 |
| RefSeq Size | 1983 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 226541 |
| UniProt ID | Q8VCK5 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209339 | Klhl20 (tGFP-tagged) - Mouse kelch-like 20 (Drosophila) (Klhl20) |
CNY 5890.00 |
|
| MR209339 | Klhl20 (Myc-DDK-tagged) - Mouse kelch-like 20 (Drosophila) (Klhl20) |
CNY 4504.00 |
|
| MR209339L3 | Lenti ORF clone of Klhl20 (Myc-DDK-tagged) - Mouse kelch-like 20 (Drosophila) (Klhl20) |
CNY 7220.00 |
|
| MR209339L4 | Lenti ORF clone of Klhl20 (mGFP-tagged) - Mouse kelch-like 20 (Drosophila) (Klhl20) |
CNY 7220.00 |
