Tcea1 (NM_011541) Mouse Untagged Clone
CAT＃: MC203335
Tcea1 (untagged) - Mouse transcription elongation factor A (SII) 1 (Tcea1), transcript variant 2, (10ug)
CNY 2400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | S-II |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC061490
CCCACGCGTCCGGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCT TCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGCCA AGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAA TATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAG AGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGAC CATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGC AAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATAT GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTG CAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGA GGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCA AATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTAT TTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGA AGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGT AAAAAGAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAATGACAACATTTGTTG TATGTAATGAATGTGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCTGGACCAT TAAGAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTTCCTTGCAAGTGAAATTT GTAAACAACATACATCTCATGGGTTGGTCTTTGTTGTTCACCTGACAGTCTGTCTTAAATGCCTTCTGTG GTCTCAGATCAGCTGGGAGACCATAAAATAATGATATAATGTGTTGCTTTGTTTTTCTCTTTCATAAGTT GATGTTGCATTTTATTAAATATTAACTTTTTATAGCCTAGAACACAAAAATTTTGTTGATCTGTTAATGC ATAAAGATAAATTGCTTTTCTCATTGGTATGTACCTAAACATGTTAAAAGGAAAAGGCATAATATAAATT TTTAGAGTTACCAAATGTAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATT TGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAGCCTCGGATCCTTGGATCTCTTGCATC TGTGACTAACCACAGTGATCATTTCTTTTATTTCTCATACATATATAATACCATTTTTTAATAGATTTTA CTCTGAAAACGGACTTGGTTTATTTTATTTGACTTTGTGCTATATTTTAGTTTTATAGAACTCTGTTATA ATTTCCTAAGCTTTTCATAAAGCCCACCACAGGTATACTTCTGATGAATTGTCCCCAATAGGAAGACATG CTATGAAGAAAATGTTAGTATCTTAGTAGAGTTCCTGGAGGCAGCATGACTGTACTTGCACTTGGAAACC TACTGACCAAGGATTAAGCCTGAGAACTGTAAATCATTAAGCTATGCTCTTTGTGTATGGAGGTTGCAAA AAAAGGCACCTCCTTTAGACTGAATCTTTTGTGCTGAAATTTTTCTTACATGATGATGTCAAGCCAGCTG AAATGTAGGCTGTAGCAAATAGTTTTACAAAACATATTTCACAATTAAGTTTTACTTTTTAAATGTGTCC CACTTGTTTTGCTAGTAATAAGCAGTGGTTTTCACATCGTCTTCCTGACACAGATGTTGTAATGCATATA GTAAAGGCCACTTTTATAAATTAAGTGCTTCTGCCTGTGTTCAGACTCCTGTGTAAAAACCGTTGCACAA GCTTTGCACTCTTCTATGCTCTGCTTTGTAGACCGTTTGTCATAACATGGAAAACAATATGTCCATGTTA AAAGGATTGGTCTGCCGGGCGTGGTGGCCCACACCTTTAATCCCAGAACTCGGGAGGCAGAGGCAGGCGG ATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACC CTGTCTCAACCCCCCTCCCCCCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAAATA AAGTACAGTTTGAAAGAATTAGTTTAAAAGTAATAAGGTTAAAATAGTCCCACTTTTAAGTGATCCATTT TAAAATTTGTAAATCAATAAAGTTTTTTGTTGTTAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_011541 |
| Insert Size | 906 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC061490, AAH61490 |
| RefSeq Size | 2576 bp |
| RefSeq ORF | 906 bp |
| Locus ID | 21399 |
| UniProt ID | P10711 |
| Gene Summary | Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204209 | Tcea1 (tGFP-tagged) - Mouse transcription elongation factor A (SII) 1 (Tcea1) |
CNY 2850.00 |
|
| MR204209 | Tcea1 (Myc-DDK-tagged) - Mouse transcription elongation factor A (SII) 1 (Tcea1), transcript variant 2 |
CNY 2400.00 |
|
| MR204209L3 | Lenti ORF clone of Tcea1 (Myc-DDK-tagged) - Mouse transcription elongation factor A (SII) 1 (Tcea1), transcript variant 2 |
CNY 4750.00 |
|
| MR204209L4 | Lenti ORF clone of Tcea1 (mGFP-tagged) - Mouse transcription elongation factor A (SII) 1 (Tcea1), transcript variant 2 |
CNY 4750.00 |
