Eif5 (NM_178041) Mouse Untagged Clone
CAT＃: MC202322
Eif5 (untagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 2, (10ug)
CNY 7504.00
货期*
现货
规格
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Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810011H21Rik; D12Ertd549e |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC042622 sequence for NM_178041
ATCGGCCTCCAACGTATCCCATGTTGACTATAACTGTCGTTTATGTCATCCCTTCTTCTCCAGACAGAAG ATACCAAAAAGTTGCAATCAAAGATCTCTTCATCTTATTGATAAAGTCACTAATAAGCCAAAATGTCTGT CAACGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTTTGATTGCTAAGGTTGAG GGCAAAGGAAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAATCGGCCTC CAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAGAATGACCG TTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTAAAAAATTT GTTCTCTGTCCTGAGTGTGAGAATCCTGAAACAGATCTGCATGTCAATCCAAAGAAGCAAACAATAGGTA ATTCTTGTAAAGCCTGTGGGTACCGAGGCATGCTTGACACACATCATAAACTCTGTACATTCATTCTCAA AAACCCACCTGAGAATAGTGACATTGGTACAGGAAAGAAAGAAAAGGAAAAGAAAAATAGAAAGGGCAAG GACAAGGAAAATGGCTCTGTATCTACCAGTGAGACACCACCACCTCCACCACCAAATGAAATTAGTCCTC CACATGCTGTGGAAGAAGAGGAAGATGATGATTGGGGGGAGGATACAACTGAGGAAGCTCAAAGGCGCAG AATGGATGAAATCAGTGACCATGCAAAAGGTCTGACACTTAGTGATGATTTGGAAAGAACTGTAGAAGAG CGTGTTAACATCCTGTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGCATTATTGATTCATCTGATAAAG AAATTGTGGCTGAGGCAGAAAGACTGGATGTAAAAGCCATGGGTCCTCTTGTTTTGACAGAAGTTCTCTT TGATGAGAAGATAAGAGAGCAAATCAAGAAATACAGGCGCCATTTTCTAAGATTTTGTCATAACAACAAA AAGGCTCAGCGGTACCTTCTTCATGGTTTGGAATGTGTGGTAGCAATGCATCAAGCTCAGCTGATCTCCA AGATCCCACACATCCTGAAGGAGATGTATGATGCCGACCTGTTAGAGGAGGAGGTCATTATCAGCTGGTC AGAAAAGGCCTCTAAGAAATATGTCTCAAAAGAACTTGCCAAAGAGATTCGTGTCAAAGCAGAGCCATTT ATTAAATGGTTGAAGGAAGCAGAGGAAGAATCTTCTGGTGGTGAGGAAGAAGATGAAGACGAAAATATTG AGGTCGTATATTCGAAAACTGCCAGTGTACCAAAAGTTGAAACTGTGAAGTCTGACAACAAGGATGATGA CATTGATATTGACGCCATTTAAAAGAATGGATGCAACTTAGCTTAACAGTGTAATGCTGCAAATTTTTCT CCATTATCAGCCAGAAGTGCAACATGTATGTGCAAGAGCTAAAGTGGCTTAACATCATGCTACACTTGAT ACTAAAAAGCTATTACTGTGAGTGGTCTATAATTAAGCCCAATGAGACATCTAGGGAGTCCATACATATC AGTGAGCAGTTGTAGTTTGCTTATTTATAGCATGTTTCTTTCGGAAAAACCTAGTGGTGGACACATTTGG ATCACATTTATACAGTTATAAAAAATAAAGATTTGATTTTGGTCATTCTTCAGACTTTGGGCTATGAATG GCTTATACTGAAGTAATTGGCTACTTTTAGGATGTTACACCATTTAATTTAGGCTCCTTGAGTTTGATGG GTTGTTAGGAACTGAAAACTTGAAGAATGCAAACAAATATAATGAACTTACTCAGCCATTAAGAAATGAA GTATTTTGAAAGTTGTATCTCCAGTCCCAGTCCATTCAGATTGGCAACTGACAATTCTTGTCATTCTAAG GAAATTGGTGATTAAATGACAGTGTGACATCCTCGTAAGAAGTAAAAATGACCTGTGTGTCCTATGGTTT AAGAGCAAATTTTAAAACTTGGAAGTTGTGGTTTTCCAGTTTGTGTACACTCACCCCCAAATTGTAGTCT TTCAAGTCGTGTGCATTGCACGTTGGATAAGCCAGGGAAATTGTTAACAAATAAGTATTTTGTGTGTGTT TAGTGGTTGCTTTGTATTGAGAGAAAAGCTTTGAGGTGTGATTAAATCGTAAGCTCTGATTCTATTTGGG AGAAACAGGAAAAAAGGTGCACTTAATCTCTAGCTAAAACAGCGTAATTTTTCAACTTTTACCCTTAAAT TATAATTTCAAGATGTTTAGACATACTGTATCTTGTGTTTGTGTACCCCCTCCCCAATATTATGGTTTAT TCTTTAATGCCTTTTAATTTGGATATAATAGCTTGTACTTTAGATTTTGGTTGCTATCTTGCCAAAATAA GTGTTACTGTTTTTCAAGCTTGACCCCCTTCCCCAATTGTCTTATTTAAAGAGAAAGTTAAACTCATACT TCTGAGTCAGAGCCTGTATTTTGGTGTAAGACTTGGGATATTTTTTACTTCACATTGAATATAGCTGGAT ACCTGAGAGACTGATGGCCACTGGGTGGGTGCAGCTAGCTAAGGCCTGACCAGCCCATTCAGAGCCTTGG ACTTCAGACACAAAAGTGAGTTCTTACACACTTGCTGGTGTAAGCTCTATCTGGGGTCCTGACTATATTT GAAAACTTGTCTTCAATATTAAAAACAGCACATATTTCTTTCTACAAAAGTGCATTCTGGGAGTTAAAAA TCCATGTGGTTAATTTGTGTGTGGCGTGCTAGCTCATACATTATTTGAATTTCATTCTTTGTGTCATCCA TCTCACAGATTATTAGACTTTAATAAACAATGTAAAAAATTGTGCATTAAAATCATACAAATATTTAGTA AAATTAGAACTTTAATAAGAGTTTGAGTGTGGAGCAGGAGCCATACACCTGGAATGGTAACAAGGGAATG TGCTGTGTCACACCAAAGATGTGGTACTTGGAAAGTCACTTGTCTCCTGGGTTTCAGATTCTTTGTTGCA TGGTCCGCCCATCTCCATTTGTCATCATTTTTTGAGATTCTCAAGTACATCAGCACATTCGGCCTCAGGT TGGCAAGATTTTTGTCTTAGAGCTGTTGCTTTAAAGGGAAACGGTCAGGTCTTAGACACTTAGGAAGGTC TTGGGCTTCTGTTCATTCTGGTGGTTAATTTGTGTGTGGCGTGCTAGCTCATACATTATTTGAATTTCAT TCTTTGTGTCATCCATCTCACAGATTATTAGACTTTAATAAACAATGTAAAAAATTGTGCATTAAAATCA TACAAATATTTAGTAAAATTAGAACTTTAATAAGAGTTTGAGTGTGGAGCAGGAGCCATACACCTGGAAT GGTAACAAGGGAATGTGCTGTGTCACACCAAAGATGTGGTACTTGGAAAGTCACTTGTCTCCTGGGTTTC AGATTCTTTGTTGCATGGTCCGCCCATCTCCATTTGTCATCATTTTTTGAGATTCTCAAGTACATCAGCA CATTCGGCCTCAGGTTGGCAAGATTTTTGTCTTAGAGCTGTTGCTTTAAAGGGAAACGGTCAGGTCTTAG ACACTTAGGAAGGTCTTGGGCTTCTGTTCATTCTGGTGCCAAACCAGTGGGATTCAAATTTCACACAATC TGGGTTTTACTCATGGAGGTTAACCTGATAGGAATAATCCTTCGTTGCTCTATTGAGGTGTCATAAAGTT TCCTGTTTCAAACAGCTACATTACTTGATTAAAACAATGTTAAAATTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_178041 |
| Insert Size | 3232 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC042622 |
| RefSeq Size | 3868 bp |
| RefSeq ORF | 3232 bp |
| Locus ID | 217869 |
| UniProt ID | P59325 |
| Gene Summary | Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses a different splice site in its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206835 | Eif5 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 2 |
CNY 3710.00 |
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