Ube2h (NM_009459) Mouse Untagged Clone
CAT＃: MC200579
Ube2h (untagged) - Mouse ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1, (10ug)
CNY 2400.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1500009C23Rik; AI181839; AI326965; AI462483; AW227540; E2-20K; Gid3; N28148; Ubc8 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC008517 sequence for NM_009459
CCCGAGCGGGCGCCTCGGTCCCCGCCCCCACTCGGCGGGGCAGTGCCCGGTGTTCCCCCGTGCGGGGGGC GGCGGCTGAGCGGACCCCACGGGACCGCGCGGGTGTTGCCACCTCGGCCGAGGAGCCGGCGAGGCCGCGG GGCCCTGCGAGCTGGGCCCGGAGCCCGTGACAGACGGGCCAAGGAGGGGAGAGAGGCGGCGGCGGCGCAG GCGACGGGGAGGCAGCGGCAGCGGCGACACCATGTCATCCCCCAGTCCCGGCAAGAGGCGGATGGACACG GACGTAGTCAAGCTCATTGAAAGCAAACATGAGGTTACAATCCTAGGAGGACTCAATGAATTCGTTGTCA AGTTTTACGGACCACAAGGAACACCATATGAAGGCGGGGTGTGGAAAGTGAGAGTGGACCTTCCTGATAA ATACCCTTTCAAGTCTCCATCTATAGGATTCATGAATAAAATTTTCCATCCCAACATTGATGAAGCGTCA GGAACTGTGTGTCTAGATGTAATTAATCAAACTTGGACAGCTCTCTATGATCTTACCAATATATTTGAGT CCTTCCTGCCCCAGTTGCTGGCCTATCCTAACCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTA CCTCCACCGACCAGAAGAGTACAAGCAGAAAATTAAAGAGTACATCCAGAAGTACGCAACGGAAGAGGCC CTGAAGGAACAGGAAGAGGGCACTGGCGACAGCTCATCGGAGAGTTCTATGTCTGACTTCTCAGAAGATG AGGCCCAGGACATGGAGTTGTAGTAGAAAAGGCATCTGCTTTTCAGAAAGACTATTATTTCCTAACCATG AGAAGCAGACTCTAATATTCATATTTAAACAAAGCAATTTTTTTTATTACTAAACAAGGTTTTTATGAAT AATAGCATTGATATATATATATTATATATCACCCTTTAGATCTTGATTTCTTGGTCATTTCTCAACCTGA GGTGCATAGCATATTCCCACATTCCATTTTGGTAGCAATATGCAGTCCGAATGCATGCATTCATAAGTCC ATTTGGCCAAGTCAGCCTGTGTGCTACTGAACTGTCAAGAGATTGCCACTCTCATAGGTAAACATGAGGA AAACAGGAAAAGTTTGCTTCTTTTTATTGATGGTTCCAAAGAAACAAACCAAATCTACCAGCTCTTGGAT GAAGATAGAACAGTGCTTTTCCAGAGTGGGAAGGAAGGGGTTGAGACGCGTGCTTTGAGAGCCTGTGAGG CTGCTCCGTCCTTGCCAGCTGCTCCCTAGTCCTGAGTGGTCCTTGGTGAGGAGCAGCAAGATGCAGCGAG TTGAGTGAGACTTAGGGCTTTTGATTTTTTTTTTTTTTTTTTTGACTCGGATTTAAGTGCATTTTTCTAG GTAGACTCCTGCCCTCCTTGCTACCAGACCTGCAGCCACCTTGGTTTTGCCTTGGAGAACATTTGGGGAG CATTTCCTGAACCCATGTCTTTTGGGGGTACAGCTTGTCATCCAGCAGGAATTATTGAGCGAGCAGGACT GGAGGAGACGGGAATGAAGACACAAGAGTGACATCTGATCCTAGCACGGGGCCTGTTGATCCATTGCCTG GACTGAGCTGTGAAACTGGACGCGGTTTTGGTTTTTTGTTTTGTGTTTCTTTTTTCTTAATTTGTGTGAA TTGTACCTAGACAGGTTACAGATTTAATTTTCACCTACCAAGGGACAGTCTCCTCCCTGGACAAGGCTCA GTGGACCATCAGATGGAGGAAGACTGGCCCCATTGCTTGAGCCCCTGTGCGACCCTTGACCTCCAGCCTT AGTTCTCCTGGAAGCTGCCAACTGCTTACGGGAGTGGGTAGATTGGGCGTCTGAGACCCAGAACATCCTA AATTTGCAATGGGTAACAGCTGAAACAAACGGAAGTTTTTGTACCAAAGTGGCCGGAGGTGGGGAAGAAC TACTTTCGAGAATTGTTACGGAGGCAGGTTGAGGGGACACTTTGACCCTCCAAGGTAACAGCCAGTCAGA TGGCTTGAGGTGCCTGGCCCATGCTGCCGGCTAAATGCCATCCAGTGTGCCCAGCAGTTTCAGACCTGAC TCTGGAGGGAATTTGGAGACAGTGTAGACAGCAGCTCTGCTCTGTATTCTTTTTGACAAAACCAGAACAG TAGAAGTAGCCAATCGTAAGACCTGAAGACATTTTGAATGTCCCACTGGACTAACCTTTCTTTTGATGTG TGTTTTTAGTTTAGAAGTGCTATATTCCACAAGGGTTAAGTCAATGAGGTTGGAAGACACCTATCTTTTT TTTTAATTCAAAAAGCACAATCAGTCTTTCCTTTGAAAATCCATATAAAGTACAACTTTTAGCGTGATTA TTTTCCTAAATACAAAGACAAATAATTTACTTATTTTATGTTAATTACGGGCATGAAGCAAAAGGTTTCT TTGGGGAACATGCAGTTTTGTAGTGCTGGGGTTGGTTCATGTCCTCTGGCACCCTGGCTTGTTGAAAGGT GGCTTTAGCTGTGTGCTGTGTACCTGCTTACGTGGCCTGCTGTCCTCTGTGTGATTAGGAGGGTTTCTGC TGGCCCTGCAGCTTGCTTCCCTGCGGATTAGGCCTTTGCTCATGCTATAGCCTTCATGTGGGTGCTACCT AGAGCAGGCGTGAACTGCTGGGTGGTGATTGTGAGTTCAGACAATAAAAAAAATGTATTTTTTTCAATAC TGTATAAAAATAGTATTCTGATTACCTCCCCCTCAATAAAAAAAAAGTCCAGATAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009459 |
| Insert Size | 552 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC008517, AAH08517 |
| RefSeq Size | 2828 bp |
| RefSeq ORF | 552 bp |
| Locus ID | 22214 |
| UniProt ID | P62257 |
| Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A conditional transposon-based insertional mutagenesis screen for hepatocellular carcinoma-associated genes in mice
,null,
Nature biotechnology
,PubMed ID 19234449
[Ube2h]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR201676 | Ube2h (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1 |
CNY 1900.00 |
|
| MR201676L3 | Lenti ORF clone of Ube2h (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1 |
CNY 3800.00 |
|
| MR201676L4 | Lenti ORF clone of Ube2h (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2H (Ube2h), transcript variant 1 |
CNY 3800.00 |
