Mouse Neurod6 (NM_009717) AAV Particle

Specifications

Product Data
orf_nt	>MR205006 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAACACTACCGTTTGACGAGTCTGTCGTAATGCCCGAATCCCAGATGTGCAGAAAGTTTGCTAGAC AATGTGAGGACCAGAAACAAATTAAGAAACCAGAGAGCTTTCCAAAACAAGTTGTCCTTCGAGGAAAGAG CATTAAAAGGGCCCCTGGAGAAGAAACCGAGAAAGAAGAGGAGGAAGAAGACAGAGAGGAAGAAGATGAG AATGGCTTGTCCAGAAGGAGGGGGCTCAGGAAAAAAAAGACCACCAAACTACGACTGGAAAGGGTCAAGT TCAGGAGACAGGAAGCTAATGCGCGCGAGAGGAACCGGATGCACGGCCTCAATGATGCTCTGGACAATTT GCGAAAAGTGGTCCCCTGTTACTCTAAAACCCAAAAACTGTCCAAAATAGAAACTTTACGACTGGCCAAA AATTACATCTGGGCACTTTCTGAAATTCTGAGGATTGGCAAGAGACCGGATCTGCTCACGTTCGTCCAAA ACTTATGCAAAGGTCTTTCCCAGCCAACTACAAACTTGGTGGCAGGCTGCTTACAGCTCAACGCCAGAAG TTTCCTGATGGGTCAGGGTGGGGAGGCTGCCCACCACACAAGGTCACCCTACTCCACATTCTACCCACCC TACCACAGCCCTGAGCTGGCCACTCCCCCAGGGCATGGGACTCTTGATAATTCCAAGTCCATGAAACCCT ACAATTACTGCAGTGCATATGAATCCTTCTATGAAAGTACCTCCCCTGAGTGTGCCAGCCCTCAGTTTGA AGGTCCCTTAAGTCCTCCCCCAATTAACTATAATGGGATATTTTCCCTGAAGCAAGAAGAAACCTTGGAC TATGGCAAAAATTACAATTATGGCATGCATTACTGTGCAGTGCCACCCAGGGGTCCCCTTGGGCAGGGTG CCATGTTCAGGTTGCCCACCGACAGCCACTTCCCTTACGACTTACATCTGCGCAGCCAATCTCTCACTAT GCAAGATGAATTAAATGCAGTTTTTCATAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
tags_filter	Myc-DDK
sub_category	AAV Particles
description	AAV ORF Particles, serotype AAV-2, Neurod6 (Myc-DDK-tagged) - Mouse neurogenic differentiation 6 (Neurod6), 250ul, >10^13 GC/mL
pdf_url	/datasheet/mr205006a1v.pdf
symbol_filter	Neurod6
cytogenetics	6 27.53 cM
purification	Iodixanol
vector	pAAV-AC-Myc-DDK
buffer	PBS with 0.001% Pluronic F68
orf_aa	>MR205006 protein sequence Red=Cloning site Green=Tags(s) MLTLPFDESVVMPESQMCRKFARQCEDQKQIKKPESFPKQVVLRGKSIKRAPGEETEKEEEEEDREEEDE NGLSRRRGLRKKKTTKLRLERVKFRRQEANARERNRMHGLNDALDNLRKVVPCYSKTQKLSKIETLRLAK NYIWALSEILRIGKRPDLLTFVQNLCKGLSQPTTNLVAGCLQLNARSFLMGQGGEAAHHTRSPYSTFYPP YHSPELATPPGHGTLDNSKSMKPYNYCSAYESFYESTSPECASPQFEGPLSPPPINYNGIFSLKQEETLD YGKNYNYGMHYCAVPPRGPLGQGAMFRLPTDSHFPYDLHLRSQSLTMQDELNAVFHN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
stability	AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
validation	To validate the quality of our AAV control particles, we have developed the following procedure: AAV genome titer is determined by qPCR. AAV transduction efficiency is analyzed by in vitro transduction in HEK293T.
serotype	AAV-2
sds_url	/sds/mr205006a1v.pdf
accn	NM_009717
seq_size	2154 bp
species_filter	Mouse
_pt	1
_c	aav-particle
synonyms	Atoh2; bHLHa2; Math-2; Math2; Nex; Nex1m
refseq	NM_009717.1
uniprot_id	P48986
mw_calculated	38.6 kDa
product_name	Mouse Neurod6 (NM_009717) AAV Particle
cell_selection	None
locus_id	11922
reforf	1014 bp
orf_size	1011 bp
category	cDNA Clones
sizes_filter	250 ul

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