Mouse Cxcl13 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse BLC Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse BLC.
Sensitivity	8pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	B-Lymphocyte Chemoattractant (BLC), also known as B Cell-Attracting chemokine 1 (BCA-1), or CXCL13, is a constitutively produced 16-18 kDa member of the α-, or CXC family of chemokines. It is synthesized as a 109 amino acid (aa) precursor that contains a 21 aa signal sequence plus an 88 aa mature segment. The mature segment contains four cysteines with no potential N-linked glycosylation sites. In the mature region, mouse BLC/BCA-1 shows only 64% aa similarity and 46% aa identity to human BLC/BCA-1. Among α-chemokines, mature BLC/BCA-1 is only 30% aa identical to mature mouse MIP-2 and KC. Like other chemokines, it is assumed that BLC forms non-disulfide linked homodimers. Cells known to express BLC are varied in type and include salivary gland epithelium, CD11b+CD11c+ dendritic cells, osteoclasts, and peritoneal macrophages.
Gene Symbol	Cxcl13
Locus ID	55985

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