Mouse Cxcl1 ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse KC Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	7.8-500 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse KC.
Sensitivity	4 pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Mouse KC, also known as CXCL1 or N51, was originally identified in fibroblasts as a PDGF-induced immediate early gene that encodes a secretory protein of approximately 8 kDa. Mouse KC cDNA encodes a 96 amino acid (aa) residue precursor protein from which the aminoterminal 19 aa residues are cleaved to generate the 77 aa residue mature KC. The protein sequence of mouse KC identified it as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines. Besides mitogen-stimulated fibroblasts, KC expression can be induced in bacterial or LPS-stimulated peritoneal and lung macrophages, endothelial cells and vascular smooth cells. The induction of KC by mitogens has been shown to be inhibited by glucocorticoids.
Gene Symbol	Cxcl1

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