Human TIMP1 ELISA KIT (96 T)

Specifications

Product Data
Description	Human TIMP-1 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human TIMP-1.
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Matrix Metalloproteinases (MMPs) are zinc-dependent endopeptidases that catalyze degradation of extracellular matrix proteins, thereby controlling such processes as development, tissue remodeling, wound healing and tumor metastasis . The activity of MMPs is controlled by regulation of expression and secretion, by proteolytic activation of pro-enzymes and by the Tissue Inhibitors of Metalloproteinases (TIMPs). TIMP-1 is a 184 amino acid residue glycosylated protein, though glycosylation is not necessary for activity . It has 12 cysteines (conserved among all TIMPs) that form disulfide bonds in a pattern that gives distinct N- and C-terminal domains . The N-terminal domain contains sites that bind to the MMP substrate-binding site . Binding of TIMP-1 does not leave a peptide bond in position for proteolysis and is not cleaved . The TIMP/MMP complex can dissociate to yield enzyme and active TIMP-1 . The C-terminal domain binds to an external site on MMPs, increasing overall affinity. TIMP-1 binds with high affinity to the inactive pro-MMP-9, forming a complex in which TIMP-1 retains its ability to inhibit the activity of another active MMP via its N-terminal domain TIMP-1 is widely synthesized by many cells and tissues . Transcription of the TIMP-1 gene is induced by pro-inflammatory cytokines (IL-1, IL-6, OSM, LIF and TNF-α), TGF-β1 and phorbol esters . Many physiological functions of TIMP-1 are closely tied to the functions of MMPs, and an improper balance of MMP and TIMP production correlates with pathological conditions such as arthritis, tumor growth and metastasis . On the other hand, TIMP-1 was independently discovered as an erythroid potentiating activity , an activity that appears to be functionally distinct from MMP inhibitory activity . TIMP-1 binds to certain cell lines and is translocated to the nucleus . It inhibits apoptosis in B cells , further suggesting that it independently functions in multiple ways to support survival and growth of cells in contrast to its function of inhibition of MMPs.
Gene Symbol	TIMP1
Locus ID	7076

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