Human PDGFA+PDGFB ELISA KIT (96 T)

Specifications

Product Data
Description	Human PDGF-AB Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human PDGF-AB.
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Human PDGF (Platelet-Derived Growth Factor) is a general term for a small group of structurallyrelated and secreted growth factors. Human PDGF-AB is a 28-30 kDa, disulfide-linked heterodimer containing one 14-16 kDa PDGF-A chain and one 12-14 kDa PDGF-B chain. Although it appears to be the first PDGF isoform recognized, its presence is suggested to be unusual in that the PDGF-A and -B chains are not commonly coexpressed. PDGF-AB has documented chemotactic properties for multiple cell types, and is also known to be mitogenic for diverse cell types. Relative to the related homodimeric isoforms, PDGF-AB will induce mitosis but will not mediate IP3 (D-myo-inositol-1,4,5-trisphosphate) production by vascular smooth muscle cells. PDGF-AA, by contrast, has no effect on either mitosis or IP3 production, while PDGF-BB induces both mitosis and IP3 production. Thus, it would appear that the three members (isoforms) of the non-CUB domain containing subfamily have some non-overlapping activities.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.