Human Fetuin A (AHSG) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Fetuin A Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Fetuin A.
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Human Fetuin A, also known as α2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene on chromosome 3. Based on the amino acid sequence similarity and conserved structure, it is classified as a member of the cystatin superfamily of cysteine protease inhibitors. Fetuin A is mainly produced by hepatocytes and monocytes/macrophages. It is most abundant in the plasma and in the bone. In the plasma, Fetuin A is a 59 kDa, disulfide bond-linked two chain polypeptide consisting of two N-terminal cystatin domains and a smaller C-terminal domain. In the bone, Fetuin A is the most abundant non-collagenous protein. It accumulates in the bone through strong binding to apatite. Fetuin A is a well-known negative acute-phase reactant, whose amounts fall in response to endogenous and exogenous stimulation.
Gene Symbol	AHSG
Locus ID	197

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