Human GRO alpha (CXCL1) ELISA KIT (96 T)

Specifications

Product Data
Description	Human CXCL1 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human CXCL1.
Sensitivity	8pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	GROα, or CXCL1, was initially discovered as a growth-regulated gene that is overexpressed constitutively in tumorigenic cells and transcribed in normal cells only during growth stimulation. The GROα cDNA encodes a 107 aa precursor protein from which the N-terminal 34 aa residues are cleaved to generate the 73 aa residue mature GROα. There are no potential N-linked glycosylation sites in the aa sequence nor does the mature protein appear to undergo any post-translational modifications. GROα exhibits growth stimulatory activity for the human Hs294T melanoma cell line from which it was initially isolated. As a chemokine α member, GROα, like IL-8, is a potent chemoattractant for human neutrophils and stimulates neutrophil degranulation and enzyme release from cytochalasin B-treated human neutrophils. It has been demonstrated that GROα can cause a decrease in the expression of interstitial collagens by rheumatoid synovial fibroblasts. In in vivo experiments, GROα has been shown to induce an acute inflammatory reaction when injected into the foot-pads of endotoxin resistant mice.
Gene Symbol	CXCL1
Locus ID	2919

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