Human Thrombopoietin (THPO) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Thrombopoietin Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	93.75-6000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Thrombopoietin.
Sensitivity	45pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,12000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Human thrombopoietin (Tpo), also known as megakaryocyte growth and development factor (MGDF), Mpl-ligand, and megakaryocyte colony stimulating factor (MK-CSF), is a 60-70 kDa, 332 amino acid (aa) residue glycosylated polypeptide that is believed to play a key role in the development of megakaryocytes. Secreted principally by hepatocytes and bone marrow stromal cells, the Tpo molecule can be structurally and functionally divided into Nand C-terminal halves. The N-terminal half of Tpo is structurally analogous to the erythropoietin (Epo) molecule, showing approximately 25% aa residue identity and conservation of a number of cysteines. Functionally, this area is suggested to be responsible for receptor binding. The C-terminal region, on the other hand, is structurally characterized by a high percentage of serine and proline residues and contains a number of potential N-linked glycosylation sites.
Gene Symbol	THPO
Locus ID	7066

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