Human GM CSF (CSF2) ELISA KIT (96 T)

Specifications

Product Data
Description	Human GM-CSF Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	4.688-300pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human GM-CSF.
Sensitivity	2pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), also known as CSF-2, is a pleiotrophic 30 kDa member of the Common beta Chain (βc) cytokine family that also includes IL-3 and IL-5. GM-CSF adopts an α-helical configuration with two intrachain disulfide bonds. It is secreted by a wide variety of activated immune, mesenchymal, and epithelial cell types and circulates as a variably glycosylated monomer. It is upregulated in multiple cell types during inflammation including encephalitogenic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF shares 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively.
Gene Symbol	CSF2
Locus ID	1437

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