Canine TNF ELISA KIT (96 T)

Specifications

Product Data
Description	Canine (dog) TNF-alpha ELISA Kit
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	34.3-2200 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant Canine TNF-a
Sensitivity	8 pg/mL
Reactivity	Canine
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2200pg/ml upon reconstitution,2 vials lyophilized, Biotin-Conjugated antibody,2 vials Concentrated Streptavidin-HRP,1 vial Standard /sample Diluent,1 bottle Biotin-Conjugate antibody Diluent,1 bottle Streptavidin-HRP Diluent,1 bottle 20 x Wash Buffer Concentrate,1 bottle Substrate Solution,1 bottle Stop Solution,1 bottle Plate Cover Seals,4 pieces
Background	TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein . Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer . Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced primarily by macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells,cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease .
Gene Symbol	TNF
Locus ID	403922

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