Human Haptoglobin (HP) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human Haptoglobin in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Haptoglobin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	3.12ng/ml-200ng/ml
Specificity	This kit is used for quantitative detection of Human Haptoglobin
Sensitivity	<20pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human Haptoglobin antibody | 1 Lyophilized recombinant human Haptoglobin standard | 200ng/tubex2 Biotinylated anti- human Haptoglobin antibody | 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family(haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans(HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.
Gene Symbol	HP

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