Rat Wif1 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of rat WIF1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for rat WIF1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	23.4pg/ml-1500pg/ml
Specificity	This kit is used for quantitative detection of rat WIF1
Sensitivity	<10pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- rat WIF1 antibody | 1 Lyophilized recombinant rat WIF1 standard | 10ng/tube×2 Biotinylated anti- rat WIF1 antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Gene Symbol	Wif1
Locus ID	114557

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