Human PRSS1/2/3 ELISA KIT (1 x 96 wells)

CAT#: EA102374

For quantitative detection of human Trypsin in cell culture supernates, serum and plasma(heparin).



CNY 5000.00


货期*
3周

规格
    • 1 x 96 wells

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Specifications

Product Data
Description For quantitative detection of human Trypsin in cell culture supernates, serum and plasma(heparin).
Size 1 x 96 wells
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for human Trypsin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Specificity This kit is used for quantitative detection of human Trypsin
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human Trypsin antibody | 1
  • Lyophilized recombinant human Trypsin standard | 10ng/tube×2
  • Biotinylated anti- human Trypsin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.
Gene Symbol PRSS1/2/3
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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