Human CD47 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human CD47 in cell culture supernates and serum.
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for human CD47
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	125pg/ml-8000pg/ml
Specificity	This kit is used for quantitative detection of human CD47
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human CD47 antibody | 1 Lyophilized recombinant human CD47 standard | 10 ng/tube×2 Biotinylated anti- human CD47 antibody | 100 μl (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100 μl (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Wash Buffer (25x) | 20 ml Adhesive cover | 4
Background	CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
Gene Symbol	CD47
Locus ID	961

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