Human TPSAB1/TPSB2 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human Tryptase in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for human Tryptase
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10000pg/ml
Specificity	This kit is used for quantitative detection of human Tryptase
Sensitivity	<15pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human Tryptase antibody | 1 Lyophilized recombinant human Tryptase standard | 10ng/tube×2 Biotinylated anti- human Tryptase antibody | 100μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Wash Buffer (25x) | 20ml Adhesive cover | 4
Background	Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
Gene Symbol	TPSAB1/TPSB2
Locus ID	7177, 64499

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