Human NBL1 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human DAN in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human DAN
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	125pg/ml-8000pg/ml
Specificity	This kit is used for quantitative detection of Human DAN
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human DAN antibody | 1 Lyophilized recombinant human DAN standard | 10ng/tube×2 Biotinylated anti- human DAN antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
Gene Symbol	NBL1
Locus ID	4681

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