HSV-2 IgG ELISA Kit

Specifications

Product Data
Description	HSV-2 IgG ELISA Kit
Size	1 kit
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum, plasma
Sample Volume	10 ul
Specificity	Natural and recombinant human HSV-2 IgG
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	The HSV 2 IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with native gG2 protein of HSV-2. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation HSV 2-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of HSV 2-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.