Human Thyroglobulin Ab (TG) ELISA kit

Specifications

Product Data
Description	Human Thyroglobulin Ab (TG) ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human Thyroglobulin Ab (TG)
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with TG antigen: 12x8x1 2. Sample Diluent: 1 bottle (ready to use): 22 ml 3. Calibrator: 1 Vial (ready to use): 1ml 4. Positive Control: 1 vial (ready to use): 1ml 5. Negative Control: 1 vial (ready to use): 1ml 6. Enzyme conjugate: 1 bottle (ready to use): 12ml 7. TMB Substrate: 1 bottle (ready to use): 12ml 8. Stop Solution: 1 bottle (ready to use): 12ml 9. Wash concentrate 20X: 1 bottle: 25ml
Background	Thyroglobulin is a water soluble glycoprotein that is involved in the storage and synthesis of thyroid hormones. The thyroid microsomal antigen has been shown to be the enzyme thyroid peroxidase (TPO). Antibodies to thyroglobulin and or microsomal antigen are present in most patients with goitrous thyroiditis (Hashimoto disease), atrophic thyroiditis and about 70-90% of Graves disease. Antibodies are also found in about half of the patients with primary hypothyroidism and thyrotoxicosis, and 10-20% of patients with simple goiters and thyroid tumors. There is also a relationship between thyroid antibodies and diabetes mellitus. Thyroid autoantibodies are present in about 6-7% of normals and their incidence increases with age. Classically, autoantibodies to thyroid antigens are detected by precipitation reactions, hemagglutination and by immunoflourescence. However the tests are subjective and lack high sensitivity. Enzyme-Linked Immunosorbent Assays (ELISAs) combine greater sensitivity, objective reading and ease of use. ELISAs have been developed and validated for detecting autoantibodies to thyroid antigens.

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