Human Secretory IgA ELISA kit

Specifications

Product Data
Description	Human Secretory IgA ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	6.25-200 µg/dl
Sample Type	Human stool or saliva
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human Secratory IgA
Sensitivity	6.25 µg/dl
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwell plate coated with anti-sIgA monoclonal Ab: 12x8x1 2. sIgA Standard: 7 vials ( ready to use): 0.125ml 3. Anti-sIgA Enzyme Conjugate: 1 vial (ready to use): 12ml 2. sIgA Bi-level Control: 2 vials (ready to use): 0.125ml 5. Incubation Buffer: 1 Bottle (Ready to use): 12ml 6. sIgA Sample Diluent: 3 Bottles: 3 x 20ml 7. TMB Substrate: 1 Bottle (ready to use): 12ml 8. Stop Solution: 1 Bottle (ready to use): 12ml 9. 20X Wash concentrate: 1 bottle: 25ml
Background	The Secretory IgA (sIgA) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-sIgA monoclonal antibody, along with the incubation buffer. After a simple washing step, an anti-SIgA enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, a chromogenic substrate (TMB) is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of sIgA in the samples. A standard curve is generated relating color intensity to the concentration of sIgA.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.