Human 25(OH) Vitamin D ELISA kit

Specifications

Product Data
Description	Human 25(OH) Vitamin D ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human 25(OH) Vitamin D
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwell plate coated with anti-Vitamin D: 12x8x1 2. Vitamin D Standard Set: 7 vials (ready to use): 0.5 ml 3. Vitamin D Control Set: 2 vials (ready to use): 0.5 ml 4. Biotinylated 25(OH)D Reagent: 1 Vial (51X): 0.55 ml 5. Assay Diluent, 1 bottle: 24 ml 6. Streptavidin-HRP, 1 bottle (ready to use): 23 mL 7. Stop Solution, 1 bottle (ready to use): 12 mL 8. TMB Substrate, 2 bottles (ready to use): 2 x 12 ml 9. Wash Concentrate 20X, 1 bottle: 25 ml
Background	Vitamin D is a steroid hormone involved in the active intestinal absorption of calcium and in the regulation of its homeostasis. Vitamin D has two isomers: Vitamin D2 and Vitamin D3. Vitamin D2 is obtained from dairy products whereas Vitamin D3 is produced in the skin after exposure to ultraviolet light. In the liver, Vitamin D is hydroxylated at its carbon 25 to form 25-OH Vitamin D. This metabolite is the predominant circulating form of Vitamin D and is considered to be an accurate indicator of the general Vitamin D status of an individual. Vitamin D deficiency has been linked to many diseases including osteoporosis, rickets, osteomalacia, cancers, and cardiovascular diseases. Both dietary supplements of Vitamin D that are currently available in the market (Vitamin D2 and Vitamin D3) are converted to 25-OH Vitamin D in the liver. The sum of the concentrations of 25-OH Vitamin D2 and 25-OH Vitamin D3, in serum or plasma, is referred to as “Total 25-OH Vitamin D”. Accurate monitoring of total 25-OH Vitamin D level is critical in clinical settings. Vitamin D deficient patients who are prescribed a daily Vitamin D supplement should regularly monitor their serum or plasma Vitamin D levels in order to reach an optimal level and prevent their 25-OH Vitamin D concentrations from reaching excessive levels that are considered toxic1-5
Standard Curve	Representative standard curve for 25(OH) Vitamin D ELISA

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