Human Thryoxine, Free (fT4) ELISA kit

Specifications

Product Data
Description	Human Thryoxine, Free (fT4) ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0.4-7.4 ng/dl
Sample Type	Serum
Sample Volume	50 µl/well
Specificity	This kit is used for quantitative detection human Thryoxine, Free (FT4)
Sensitivity	0.4 ng/dl
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with Streptavidin: 12x8x1 2. fT4 Standard: 6 vials ( ready to use): 0.5 ml 3. Anti-T4 Biotin Solution: 7 ml 4. fT4 Enzyme conjugate: 1 Bottle (ready to use): 7 ml 5. TMB Substrate: 1 bottle (ready to use): 12 ml 6. Stop Solution: 1 bottle (ready to use): 12 ml 7. 20X Wash concentrate: 1 bottle: 25 ml
Background	Over 99% of thyroxine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of Thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
Standard Curve	Representative standard curve for Thryoxine, Free (FT4) ELISA

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