Human Progranulin ELISA Kit

Specifications

Product Data
Description	Human Progranulin ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Progranulin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	1.56ng/ml-100ng/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human Progranulin
Sensitivity	<50pg/ml
Reactivity	Human
Cross Reactivity	This kit is for the detection of Human GRN. No significant cross-reactivity or interference between GRN and its analogs was observed. This claim is limited by existing techniques therefore cross-reactivity may exist with untested analogs.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human Progranulin antibody | 1 Lyophilized recombinant human Progranulin standard | 100ng/tube x 2 Biotinylated anti- human Progranulin antibody |100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) |100 ul (dilution 1:100) Sample diluent buffer| 30 ml Antibody diluent buffer| 12ml ABC diluent buffer| 12ml TMB color developing agent| 10ml TMB stop solution |10ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Gene Symbol	GRN

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