Human THBS1/TSP1 ELISA Kit

Specifications

Product Data
Description	Human THBS1/TSP1 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human THBS1/TSP1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	1.56 ng/ml-100 ng/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human THBS1
Sensitivity	<50pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human THBS1/TSP1 antibody 1 Lyophilized recombinant human THBS1/TSP1 standard 100 ng/tubex2 Biotinylated anti- human THBS1/TSP1 antibody 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100 ul (dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12 ml ABC diluent buffer 12 ml TMB color developing agent 10 ml TMB stop solution 10 ml Wash Buffer (25x) 20 ml Plate Sealers 4 Piece
Background	Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Gene Symbol	THBS1
Locus ID	7057

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