Human PLAT/TPA ELISA Kit

Specifications

Product Data
Description	Human PLAT/TPA ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human PLAT/TPA
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human PLAT
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human PLAT antibody 1 Lyophilized recombinant human PLAT standard 10ng/tubex2 Biotinylated anti- human PLAT antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Gene Symbol	PLAT
Locus ID	5327

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