Human NT-4 ELISA Kit

Specifications

Product Data
Description	Human NT-4 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human NT-4
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Sample Type	Cell culture supernates and serum.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human NT-4
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human NT-4 antibody 1 Lyophilized recombinant human NT-4 standard 10ng/tubex2 Biotinylated anti- human NT-4 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.
Gene Symbol	NTF4
Locus ID	4909
Standard Curve	Representative standard curve for Human NT-4 PicoKine ELISA Kit

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