Mouse IL-25(IL-17E) ELISA Kit (96-well)

Specifications

Product Data
Description	Mouse IL-25(IL-17E) ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.03 - 2.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Mouse IL-25(IL-17E) Ligand
Sensitivity	15pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-25/IL-17E (8 x 12) 2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/vial upon reconstitution 2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	IL-25 (also known as IL-17E) is a member of the IL-17 family of proinflammatory cytokines, which includes IL-17A, IL-17B, IL-17C, IL-17F and IL-17A/F. IL-17E is found at low levels in various peripheral tissues. It is a proinflammatory cytokine that promotes airway eosinophilia in mice. IL-17E plays a role in Th2 type inflammatory diseases, such as asthma and atopic dermatitis. IL-25 induces elevatedgene expression of IL-4, IL-5 and IL-13 in multiple tissues and results in T helper 2 (TH2)-type immune responses (increased serumIgE levels) and pathologic changes in the lungsand digestive tract with eosinophilic infiltrates, increased mucusproduction, epithelial cell hyperplasia and overall amplified allergicinflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.
Gene Symbol	Il25
Locus ID	140806
Standard Curve	Representative standard curve for IL-25/IL-17E ELISA. IL-25/IL-17E was diluted in serial two-fold steps in Sample Diluent.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.