Human OSM-R ELISA Kit (96-well)

Specifications

Product Data
Description	Human OSM-R ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	312.5-20000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human OSM-R Ligand
Sensitivity	80pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human OSM-R (8X12) 2 vials human OSM-R Standard lyophilized, 10000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human OSM-R antibody 2 vials Streptavidin-HRP solution 4 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	OSM is a cytokine originally isolated from medium conditioned by PMAtreated U 937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid preproOSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic Cterminaldomain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and proOSM are equally active in radioreceptor assays, the mature OSM is 5to 60 fold more active in growth inhibition assays. Thus, proteolytic processing of the proOSM peptide may be important in regulating the in vivo activities of OSM. OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component (. subunit) of the IL6, LIF and CNTF receptor complexes, was identified as a lowaffinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130related protein) has now been identified to be a component of a highaffinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDSKaposiÂ?s sarcoma cells, and a human erythroleukemia cell line, TF1. Other OSMmediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells. regulation of IL6 expression in human endothelial cells. and stimulation of LDL uptake and upregulation of cell surface LDL receptors in HepG2 cells.
Gene Symbol	OSMR
Standard Curve	Representative standard curve for OSM-R ELISA. OSM-R was diluted in serial two-fold steps in Sample Diluent.

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