Human TNF-a ELISA Kit (96-well)

Specifications

Product Data
Description	Human TNF-a ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human TNF-a Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human TNF-a(8x12) 2 vials human TNF-a Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TNF-a antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	The prototype ligand of the TNF superfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis . Human cells known to express TNF-. include B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes. It is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids (aa) in length . It contains a 30 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region . TNF-. is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-. (sTNF-. is released from the C-terminus of the transmembrane protein through the activity of TNF-.-converting enzyme (TACE), a membrane-bound disintegrin metalloproteinase . TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells . TNF-. has been implicated in a number of pathophysiological processes. It is associated with unregulated pro-inflammatory activity and is thought to be a critical mediator of endotoxin-induced septic shock . Cachexia (or whole body wasting) has also been associated with long-term circulating TNF-.. Other disorders with potential TNF-. involvement include asthma), type 2 diabetes, CrohnÂ?s disease, and rheumatoid arthritis.
Gene Symbol	TNF
Locus ID	7124

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