IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425

Specifications

Product Data
Product Name	IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
Clone Name	PHE425
Applications	ELISA, IHC, WB
Recommend Dilution	Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user.
Reactivity	Porcine
Host	Mouse
Immunogen	Porcine liver extract.
Formulation	Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.