Rat IgG (H+L chain) F(ab')2 Fragment Donkey Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG (H+L chain) F(ab')2 Fragment Donkey Polyclonal Antibody
Recommend Dilution	Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivity	Rat
Host	Donkey
Immunogen	Rat IgG whole molecule
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride; pH 7.2 with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer. Label: PE State: Lyophilized F(ab')2 fragments. Label: R-Phycoerythrin ( isolated from a culture of red algae. Subunit structure - (αβ)6γ Apparent Molecular Weight - 240 kDa (alpha 20 kDa, beta 20 kDa, gamma 30 kDa). Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound) Absorption emission: 490 nm, 545 nm and 565 nm / 580 nm
Purification	Immunoaffinity chromatography.
Conjugation	PE
Storage Condition	Store the antibody (undiluted) at 2-8°C prior to opening. DO NOT FREEZE! Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Dilute only prior to immediate use.

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