Canine IgG (Fc specific) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Canine IgG (Fc specific) Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:44,000 of the reconstitution concentration is suggested for this product.
Reactivity	Dog
Host	Rabbit
Immunogen	Dog IgG F(c) fragment
Formulation	0.05M Tris Chloride, 0.15 M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer. Label: AP State: Liquid (sterile filtered) purified Ig fraction Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	AP
Storage Condition	Store vial at 2-8°C. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.

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