Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

Specifications

Product Data
Product Name	Rabbit IgG (H+L chain) Sheep Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500.
Reactivity	Rabbit
Host	Sheep
Immunogen	Rabbit IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase Molar radio: F/P (OD403/OD280) = 0.471
Concentration	lot specific
Purification	Immunoaffinity Chromatography using Rabbit IgG coupled to agarose.
Conjugation	HRP
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with deioinzed water (see 'Format'). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.