Mouse IgG (H+L chain) Chicken Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (H+L chain) Chicken Polyclonal Antibody
Applications	ELISA, ID, WB
Recommend Dilution	ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivity	Mouse
Host	Chicken
Immunogen	Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.
Formulation	10 mM Sodium Phosphate, pH 7.2, 0.15 M Sodium Chloride Label: HRP State: Liquid purified Ig fraction. Stabilizer: 10 % (w/v) BSA (Protease and IgG free) Preservative: 0.1 % (v/v) Kathon CG Label: Horseradish Peroxidase
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	HRP
Storage Condition	Store the antibody undiluted at 2-8°C in the dark.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.