Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
Applications	IF, IHC, IP, WB
Recommend Dilution	ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution). Western blot (1/5000). Immunocytochemistry. Immunohistochemistry (1/250). Immunoprecipitation (1/250). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivity	Chicken
Host	Goat
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Liquid purified IgY fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C in the dark for one month or Add Sterile Glycerol and store at -20°C for longer. Avoid repeated freezing and thawing. Centrifuge vial before opening.

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