Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]

Specifications

Product Data
Product Name	Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
Clone Name	NI 132 (HP 6186)
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. General Recommended Dilutions: Immunocytochemistry. Immunohistochemistry (Paraffin). Histochemical Use: 1/10-1/40. ELISA: 1/100-1/2000.  Western blot: 1/200-1/4000.
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgG1 isolated from pooled Human serum.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins Label: Biotin State: Lyophilized purified Ig fraction Molar radio: Biotin/IgG ~4.2
Concentration	0.4 mg/ml
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.