TLE 1 (TLE1) Mouse Monoclonal Antibody [Clone ID: UMAB253]

Specifications

Product Data
Clone Name	UMAB253
Applications	10k-ChIP, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:100~300, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	83 kDa
Gene Name	TLE family member 1, transcriptional corepressor
Database Link	NP_005068 Entrez Gene 21885 MouseEntrez Gene 362533 RatEntrez Gene 7088 Human UniProt ID Q04724
Synonyms	ESG; ESG1; GRG1
Reference Data
Protein Families	Transcription Factors

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