GATA3 Mouse Monoclonal Antibody [Clone ID: UMAB218]

Specifications

Product Data
Clone Name	UMAB218
Applications	10k-ChIP, IF, IHC, WB
Recommend Dilution	IHC 1:50~100, IF 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	47.9 kDa
Gene Name	GATA binding protein 3
Database Link	NP_001002295 Entrez Gene 14462 MouseEntrez Gene 85471 RatEntrez Gene 2625 Human UniProt ID P23771
Background	This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Synonyms	HDR; HDRS
Reference Data
Protein Families	Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors

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