LGR5 Mouse Monoclonal Antibody [Clone ID: UMAB212]

Specifications

Product Data
Clone Name	UMAB212
Applications	10k-ChIP, FC, IF, IHC, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:100~150 (recommended antigen retrieval buffer: TEE buffer pH 9.0), IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	99.8 kDa
Gene Name	leucine rich repeat containing G protein-coupled receptor 5
Database Link	NP_003658 Entrez Gene 14160 MouseEntrez Gene 299802 RatEntrez Gene 8549 Human UniProt ID O75473
Synonyms	FEX; GPR49; GPR67; GRP49; HG38
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane

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