NCF4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI12H10]

Specifications

Product Data
Clone Name	OTI12H10
Applications	IHC
Recommend Dilution	IHC 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NCF4 (NP_000622) produced in HEK293T cell.
Isotype	IgG1
Formulation	PBS (pH 7.3) containing 1% BSA, 50% glycerol.
Concentration	0.5 mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	HRP
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.9 kDa
Gene Name	neutrophil cytosolic factor 4
Database Link	NP_000622 Entrez Gene 17972 MouseEntrez Gene 500904 RatEntrez Gene 4689 Human UniProt ID Q15080
Background	The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Synonyms	NCF; P40PHOX; SH3PXD4
Reference Data
Protein Pathways	Leukocyte transendothelial migration

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